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Article Dans Une Revue Life Science Alliance Année : 2023

Palmitate impairs circadian transcriptomics in muscle cells through histone modification of enhancers

Laura Sardón Puig
  • Fonction : Auteur
Ali Altıntaş
Prasad Kamble
  • Fonction : Auteur
Salvador Casaní-Galdón
  • Fonction : Auteur
Brendan Gabriel
Alexander Chibalin
Anna Krook
  • Fonction : Auteur
Juleen Zierath

Résumé

Obesity and elevated circulating lipids may impair metabolism by disrupting the molecular circadian clock. We tested the hypothesis that lipid overload may interact with the circadian clock and alter the rhythmicity of gene expression through epigenomic mechanisms in skeletal muscle. Palmitate reprogrammed the circadian transcriptome in myotubes without altering the rhythmic mRNA expression of core clock genes. Genes with enhanced cycling in response to palmitate were associated with post-translational modification of histones. The cycling of histone 3 lysine 27 acetylation (H3K27ac), a marker of active gene enhancers, was modified by palmitate treatment. Chromatin immunoprecipitation and sequencing confirmed that palmitate exposure altered the cycling of DNA regions associated with H3K27ac. The overlap between mRNA and DNA regions associated with H3K27ac and the pharmacological inhibition of histone acetyltransferases revealed novel cycling genes associated with lipid exposure of primary human myotubes. Palmitate exposure disrupts transcriptomic rhythmicity and modifies enhancers through changes in histone H3K27 acetylation in a circadian manner. Thus, histone acetylation is responsive to lipid overload and may redirect the circadian chromatin landscape, leading to the reprogramming of circadian genes and pathways involved in lipid biosynthesis in skeletal muscle.

Dates et versions

hal-04311636 , version 1 (28-11-2023)

Identifiants

Citer

Nicolas Pillon, Laura Sardón Puig, Ali Altıntaş, Prasad Kamble, Salvador Casaní-Galdón, et al.. Palmitate impairs circadian transcriptomics in muscle cells through histone modification of enhancers. Life Science Alliance, 2023, 6 (1), pp.e202201598. ⟨10.26508/lsa.202201598⟩. ⟨hal-04311636⟩
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