Communication Dans Un Congrès Année : 2023

Hydroxycinnamic acid amide accumulation and PR-protein encoding gene expressionare major responses of wheat during the early stages of powdery mildew infection

Ali Siah

Résumé

Blumeria graminis f.sp. tritici (Bgt) is an obligate biotrophic fungal pathogen responsible for powdery mildew in bread wheat (Triticum aestivum L.). During the firststeps of this pathogenic interaction, basal defense mechanisms take place in wheat leaves. We used RT-qPCR and metabolomic approaches to analyze these earlystages of the interaction between Bgt and the moderately susceptible wheat cultivar Pakito. The expression of genes encoding pathogenesis-related (PR) proteins (PR1,PR4, PR5 and PR8), known to target the pathogen, increased during the first 48 hours post-inoculation. Moreover, RT-qPCR and metabolomic analyses pointed out theimportance of the phenylpropanoid pathway in quantitative resistance against Bgt. Among metabolites of this pathway, hydroxycinnamic acid amides containing agmatineand putrescine as amine components accumulated from the second to the fourth day after inoculation. This suggests the involvement of these metabolites in quantitativeresistance via cross-linking processes in cell wall for reinforcement, what is supported by the up-regulation of PAL (phenylalanine ammonia-lyase), PR15 (encoding anoxalate oxidase) and POX (peroxidase) further inoculation. This study increases knowledge on basal resistance in wheat, giving new targets to investigate inducedresistance in wheat towards Bgt.

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Dates et versions

hal-04309318 , version 1 (27-11-2023)

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  • HAL Id : hal-04309318 , version 1

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Thierry Allario, Alice Fourquez, Maryline Magnin-Robert, Ali Siah, Alessandra Maia-Grondard, et al.. Hydroxycinnamic acid amide accumulation and PR-protein encoding gene expressionare major responses of wheat during the early stages of powdery mildew infection. The 12th International Congress of Plant Pathologie (ICPP 2023) : "ONE HEALTH for all Plants, Crops and Trees”, Nathalie Poussereau - laboratoire MAP; Mathias Choquer - laboratoire MAP, Aug 2023, Lyon, France. ⟨hal-04309318⟩
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