Cytoskeleton Remodeling in Arabidopsis Stigmatic Cells Following Pollination - Archive ouverte HAL
Chapitre D'ouvrage Année : 2023

Cytoskeleton Remodeling in Arabidopsis Stigmatic Cells Following Pollination

Résumé

In plants, the first interaction that occurs between the male gametophytes (pollen grains) and the stigmatic epidermis of the female organ is crucial for successful reproduction. The stigma consists of a dome of flask-shaped cells specialized in pollen capture. In these stigmatic cells, the cytoskeleton network (cortical microtubules and actin microfilaments) actively responds to pollen contact and undergoes dynamic remodeling required for successful pollen acceptance to occur. Here, we have designed several microscopy mountings to monitor stigmatic cytoskeleton dynamics. These designs are based on the constraints linked to the tightly regulated pollen-stigma interaction and depend upon the experimental goal, either a static view or live-cell imaging.
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hal-04302980 , version 1 (16-07-2024)

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Lucie Riglet, Isabelle Fobis-Loisy. Cytoskeleton Remodeling in Arabidopsis Stigmatic Cells Following Pollination. Patrick J. Hussey; Pengwei Wang. The Plant Cytoskeleton. Methods and Protocols, 2604, Springer US, pp.215-225, 2023, Methods in Molecular Biology, 978-1-0716-2869-0. ⟨10.1007/978-1-0716-2867-6_16⟩. ⟨hal-04302980⟩
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