Silicon chambers for enhanced incubation and imaging of microfluidic droplets - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Lab on a Chip Année : 2023

Silicon chambers for enhanced incubation and imaging of microfluidic droplets

Résumé

Droplet microfluidics has become a powerful tool in life sciences, underlying digital assays, single-cell sequencing or directed evolution, and it is making foray in physical sciences as well. Imaging and incubation of droplets are crucial, yet they are encumbered by the poor optical, thermal and mechanical properties of PDMS, a material commonly used in microfluidics labs. Here we show that Si is an ideal material for droplet chambers. Si chambers pack droplets in a crystalline and immobile monolayer, are immune to evaporation or sagging, boost the number of collected photons, and tightly control the temperature field sensed by droplets. We use the mechanical and optical benefits of Si chambers to image ≈1 million of droplets from a multiplexed digital assay – with an acquisition rate similar to the best in-line methods. Lastly, we demonstrate their applicability with a demanding assay that maps the thermal dependence of Michaelis–Menten constants with an array of ≈150 000 droplets. The design of the Si chambers is streamlined to avoid complicated fabrication and improve reproducibility, which makes Si a complementary material to PDMS in the toolbox of droplet microfluidics.
Fichier sous embargo
Fichier sous embargo
0 0 14
Année Mois Jours
Avant la publication
dimanche 12 mai 2024
Fichier sous embargo
dimanche 12 mai 2024
Connectez-vous pour demander l'accès au fichier

Dates et versions

hal-04297955 , version 1 (21-11-2023)

Identifiants

Citer

Nicolas Lobato-Dauzier, Robin Deteix, Guillaume Gines, Alexandre Baccouche, Benediktus Nixon Hapsianto, et al.. Silicon chambers for enhanced incubation and imaging of microfluidic droplets. Lab on a Chip, 2023, 23 (12), pp.2854-2865. ⟨10.1039/D2LC01143C⟩. ⟨hal-04297955⟩
16 Consultations
1 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More