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Article Dans Une Revue Biochimica et Biophysica Acta:Biomembranes Année : 2023

Incorporation and localisation of alkanes in a protomembrane model

Alkane localisation inside a compliant protomembrane model

Résumé

Protomembranes at the origin of life were likely composed of short-chain lipids, readily available on the early Earth. On the other hand, membranes formed by such lipids are less stable and more permeable under extreme conditions, so that a novel membrane architecture was suggested to validate the accuracy of this assumption. The model membrane includes the presence of a layer of alkanes in the mid-plane of the protomembrane in between the two monolayer leaflets and lying perpendicular to the lipid acyl chains. Here, we investigated such possibility experimentally for membranes formed by the short-chain phospholipid 1,2-didecanoyl-sn-glycero-3-phophocholine, including or not the alkanes eicosane, squalane or triacontane by means of neutron membrane diffraction and contrast variation. We found strong indications for an incorporation of two of the three alkanes in the membrane mid-plane through the determination of neutron scattering length density profiles and the membrane swelling at various relative humidities indicating a slightly increased bilayer thickness when the alkanes are incorporated into the bilayers. The selectivity of the incorporation points out the role of the length of the n-alkanes with respect to the capacity of the membrane to incorporate them.
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Dates et versions

hal-04297309 , version 1 (21-11-2023)

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Loreto Misuraca, Josephine Loricco, Philippe Oger, Judith Peters, Bruno Demé. Incorporation and localisation of alkanes in a protomembrane model. Biochimica et Biophysica Acta:Biomembranes, 2023, 1865 (3), pp.184119. ⟨10.1016/j.bbamem.2023.184119⟩. ⟨hal-04297309⟩
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