Exploring the World of Membrane Proteins: Techniques and Methods for Understanding Structure, Function, and Dynamics - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecules Année : 2023

Exploring the World of Membrane Proteins: Techniques and Methods for Understanding Structure, Function, and Dynamics

Imad Boulos
  • Fonction : Auteur
  • PersonId : 1312249
Joy Jabbour
  • Fonction : Auteur
  • PersonId : 1312250
Serena Khoury
  • Fonction : Auteur
  • PersonId : 1312251
Nehme Mikhael
  • Fonction : Auteur
  • PersonId : 1312252
Hilda E Ghadieh
  • Fonction : Auteur
  • PersonId : 1312253
Youssef Bassim
  • Fonction : Auteur
  • PersonId : 1312254
Sami Azar
  • Fonction : Auteur
  • PersonId : 1312255
Frédéric Harb

Résumé

In eukaryotic cells, membrane proteins play a crucial role. They fall into three categories: intrinsic proteins, extrinsic proteins, and proteins that are essential to the human genome (30% of which is devoted to encoding them). Hydrophobic interactions inside the membrane serve to stabilize integral proteins, which span the lipid bilayer. This review investigates a number of computational and experimental methods used to study membrane proteins. It encompasses a variety of technologies, including electrophoresis, X-ray crystallography, cryogenic electron microscopy (cryo-EM), nuclear magnetic resonance spectroscopy (NMR), biophysical methods, computational methods, and artificial intelligence. The link between structure and function of membrane proteins has been better understood thanks to these approaches, which also hold great promise for future study in the field. The significance of fusing artificial intelligence with experimental data to improve our comprehension of membrane protein biology is also covered in this paper. This effort aims to shed light on the complexity of membrane protein biology by investigating a variety of experimental and computational methods. Overall, the goal of this review is to emphasize how crucial it is to understand the functions of membrane proteins in eukaryotic cells. It gives a general review of the numerous methods used to look into these crucial elements and highlights the demand for multidisciplinary approaches to advance our understanding.
Fichier principal
Vignette du fichier
Harb_mb_protein_rev.pdf (690.05 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : CC BY - Paternité

Dates et versions

hal-04296457 , version 1 (20-11-2023)

Licence

Paternité

Identifiants

Citer

Imad Boulos, Joy Jabbour, Serena Khoury, Nehme Mikhael, Victoria Tishkova, et al.. Exploring the World of Membrane Proteins: Techniques and Methods for Understanding Structure, Function, and Dynamics. Molecules, 2023, 28 (20), pp.7176. ⟨10.3390/molecules28207176⟩. ⟨hal-04296457⟩
15 Consultations
10 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More