Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish? - Archive ouverte HAL
Article Dans Une Revue Science Advances Année : 2023

Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?

Résumé

The constant exposure of the fish branchial cavity to aquatic pathogens causes local mucosal immune responses to be extremely important for their survival. Here, we used a marker for T lymphocytes/natural killer (NK) cells (ZAP70) and advanced imaging techniques to investigate the lymphoid architecture of the zebrafish branchial cavity. We identified a sub-pharyngeal lymphoid organ, which we tentatively named "Nemausean lymphoid organ" (NELO). NELO is enriched in T/NK cells, plasma/B cells, and antigen-presenting cells embedded in a network of reticulated epithelial cells. The presence of activated T cells and lymphocyte proliferation, but not V(D)J recombination or hematopoiesis, suggests that NELO is a secondary lymphoid organ. In response to infection, NELO displays structural changes including the formation of T/NK cell clusters. NELO and gill lymphoid tissues form a cohesive unit within a large mucosal lymphoid network. Collectively, we reveal an unreported mucosal lymphoid organ reminiscent of mammalian tonsils that evolved in multiple teleost fish familie

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hal-04294675 , version 1 (20-11-2023)

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Julien Resseguier, Maï E. Nguyen-Chi, Jens Wohlmann, Dimitri Rigaudeau, Irene Salinas, et al.. Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?. Science Advances , 2023, 9 (44), pp.eadj0101. ⟨10.1126/sciadv.adj0101⟩. ⟨hal-04294675⟩
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