Characterization of polarization dependence in super-resolution fluorescent microscopy via phase retrieval - Archive ouverte HAL
Pré-Publication, Document De Travail Année : 2023

Characterization of polarization dependence in super-resolution fluorescent microscopy via phase retrieval

Isael Herrera
  • Fonction : Auteur
Sophie Brasselet
Miguel Alonso
  • Fonction : Auteur

Résumé

In single molecule orientation localization microscopy, aberrations cause changes in the shape of the point spread function (PSF) generated by point-like dipolar sources, which can lead to an erroneous estimation of the source's position and orientation. A common strategy for addressing this issue is to model the aberrations as a scalar pupil phase mask and characterize them using a stack of PSFs for varying defocus using phase retrieval algorithms. However, this strategy fails when there are polarization-dependent aberrations, introduced either through unintentional anisotropy in the system or by using birefringent masks for PSF shaping. Here, a model and methodology for the proper characterization of polarization-dependent aberrations are introduced. The key components are the modeling of polarization aberrations via a spatially-dependent Jones matrix, commonly used to describe birefringent elements, and the introduction of polarization diversity for its correct estimation via a phase retrieval algorithm based on a nonlinear optimization. The software pyPSFstack used for the modeling and characterization is also made freely available.
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Dates et versions

hal-04286795 , version 1 (15-11-2023)

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Rodrigo Gutiérrez-Cuevas, Luis Alemán-Castañeda, Isael Herrera, Sophie Brasselet, Miguel Alonso. Characterization of polarization dependence in super-resolution fluorescent microscopy via phase retrieval. 2023. ⟨hal-04286795⟩
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