G-quadruplexes are promoter elements controlling nucleosome exclusion and RNA polymerase II pausing - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2023

G-quadruplexes are promoter elements controlling nucleosome exclusion and RNA polymerase II pausing

Résumé

Despite their central role in transcription, it has been difficult to define universal sequences associated to eukaryotic promoters. Within chromatin context, recruitment of the transcriptional machinery requires opening of the promoter but how DNA elements could contribute to this process has remained elusive. Here, we show that G-quadruplex (G4) secondary structures are highly enriched mammalian core promoter elements. G4s are located at the deepest point of nucleosome exclusion at promoters and correlate with maximum promoter activity. We found that experimental G4s exclude nucleosomes both in vivo and in vitro and display a strong positioning potential. At model promoters, impairing G4s affected both transcriptional activity and chromatin opening. G4 destabilization also resulted in an inactive promoter state and affected transition to effective RNA production in live imaging experiments. Finally, G4 stabilization resulted in global reduction of proximal promoter pausing. Altogether, our data introduce G4s as bona fide promoter elements allowing nucleosome exclusion and facilitating pause release by the RNA Polymerase II.
Fichier principal
Vignette du fichier
15_Esnault_et_al_BioRxiv_2023.pdf (9.88 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04286714 , version 1 (15-11-2023)

Identifiants

Citer

Cyril Esnault, Encar Garcia-Oliver, Amal Zine El Aabidine, Marie-Cécile Robert, Talha Magat, et al.. G-quadruplexes are promoter elements controlling nucleosome exclusion and RNA polymerase II pausing. 2023. ⟨hal-04286714⟩
25 Consultations
27 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More