Tho2 is critical for the recruitment of Rrp6 to chromatin in response to perturbed mRNP biogenesis
Résumé
The eukaryotic THO complex coordinates the assembly of so-called messenger RNA-ribonucleoprotein particles (mRNPs), a process that involves co-transcriptional coating nascent mRNAs with proteins. Once formed, mRNPs undergo quality control step marks them either for active transport to cytoplasm, or Rrp6/RNA exosome-mediated degradation in nucleus. However, mechanism behind is still unclear. We investigated budding yeast by expressing bacterial Rho helicase, which globally perturbs mRNP formation. examined genome-wide binding profiles subunits Tho2, Thp2, Hpr1, and Mft1 upon perturbation biogenesis, found Tho2 plays two roles. In addition its function as subunit complex, biogenesis targets Rrp6 chromatin via C-terminal domain. Interestingly, other are not enriched on necessary localizing at target loci. Our study highlights potential role control, independent subunits. Considering both RNA exosome evolutionarily highly conserved, our findings likely relevant surveillance mammals.
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