Article Dans Une Revue Regenerative Biomaterials Année : 2020

Collagen scaffolds functionalized with triple-helical peptides support 3D HUVEC culture

Emma Hunter
  • Fonction : Auteur
Natalia Davidenko
  • Fonction : Auteur
Daniel Bax
  • Fonction : Auteur
Ruth Cameron
  • Fonction : Auteur
Serena Best
  • Fonction : Auteur
Sanjay Sinha
  • Fonction : Auteur
Richard Farndale
  • Fonction : Auteur

Résumé

Abstract Porous biomaterials which provide a structural and biological support for cells have immense potential in tissue engineering and cell-based therapies for tissue repair. Collagen biomaterials that can host endothelial cells represent promising tools for the vascularization of engineered tissues. Three-dimensional collagen scaffolds possessing controlled architecture and mechanical stiffness are obtained through freeze–drying of collagen suspensions, followed by chemical cross-linking which maintains their stability. However, cross-linking scaffolds renders their biological activity suboptimal for many cell types, including human umbilical vein endothelial cells (HUVECs), by inhibiting cell–collagen interactions. Here, we have improved crucial HUVEC interactions with such cross-linked collagen biomaterials by covalently coupling combinations of triple-helical peptides (THPs). These are ligands for collagen-binding cell-surface receptors (integrins or discoidin domain receptors) or secreted proteins (SPARC and von Willebrand factor). THPs enhanced HUVEC adhesion, spreading and proliferation on 2D collagen films. THPs grafted to 3D-cross-linked collagen scaffolds promoted cell survival over seven days. This study demonstrates that THP-functionalized collagen scaffolds are promising candidates for hosting endothelial cells with potential for the production of vascularized engineered tissues in regenerative medicine applications.

Dates et versions

hal-04285734 , version 1 (14-11-2023)

Identifiants

Citer

Jean-Daniel Malcor, Emma Hunter, Natalia Davidenko, Daniel Bax, Ruth Cameron, et al.. Collagen scaffolds functionalized with triple-helical peptides support 3D HUVEC culture. Regenerative Biomaterials, 2020, 7 (5), pp.471-482. ⟨10.1093/rb/rbaa025⟩. ⟨hal-04285734⟩
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