The Antibody Dependant Neurite Outgrowth Modulation Response Involvement in Spinal Cord Injury - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Immunology Année : 2022

The Antibody Dependant Neurite Outgrowth Modulation Response Involvement in Spinal Cord Injury

Alice Capuz
  • Fonction : Auteur
Mélodie-Anne Karnoub
  • Fonction : Auteur
Sylvain Osien
  • Fonction : Auteur
Mélanie Rose
  • Fonction : Auteur
Céline Mériaux
  • Fonction : Auteur
Isabelle Fournier
David Devos
Franck Rodet
Dasa Cizkova
  • Fonction : Auteur
Michel Salzet

Résumé

Spinal cord injury (SCI) represents a major medical challenge. At present, there is still no cure to treat it efficiently and enable functional recovery below the injury site. Previously, we demonstrated that inflammation determines the fate of the physiopathology. To decipher the molecular mechanisms involved in this process, we performed a meta-analysis of our spatio-temporal proteomic studies in the time course of SCI. This highlighted the presence of IgG isotypes in both spinal cord explants and their secretomes. These IgGs were detected in the spinal cord even if no SCI occurred. However, during the time course following SCI, abundance of IgG1 and IgG2 subclasses (a, b, c) varied according to the spatial repartition. IgG1 was clearly mostly abundant at 12 h, and a switch to IgG2a was observed after 24 h. This IgG stayed predominant 3, 7, and 10 days after SCI. A protein related to IgM as well as a variable heavy chain were only detected 12 h after lesion. Interestingly, treatment with RhoA inhibitor influenced the abundance of the various IgG isotypes and a preferential switch to IgG2c was observed. By data reuse of rat dorsal root ganglion (DRG) neurons RNAseq datasets and RT-PCR experiments performed on cDNA from DRG sensory neurons ND7/23 and N27 dopaminergic neural cell lines, we confirmed expression of immunoglobulin heavy and light chains (constant and variable) encoding genes in neurons. We then identified CD16 and CD32b as their specific receptors in sensory neuron cell line ND7/23 and their activation regulated neurites outgrowth. These results suggest that during SCI, neuronal IgG isotypes are released to modulate neurites outgrowth. Therefore, we propose a new view of the SCI response involving an antibody dependent neurite outgrowth modulation (ADNM) which could be a precursor to the neuroinflammatory response in pathological conditions.

Dates et versions

hal-04278167 , version 1 (09-11-2023)

Identifiants

Citer

Alice Capuz, Mélodie-Anne Karnoub, Sylvain Osien, Mélanie Rose, Céline Mériaux, et al.. The Antibody Dependant Neurite Outgrowth Modulation Response Involvement in Spinal Cord Injury. Frontiers in Immunology, 2022, 13, ⟨10.3389/fimmu.2022.882830⟩. ⟨hal-04278167⟩

Collections

UNIV-LILLE
32 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More