Real-time in vivo ROS monitoring with luminescent nanoparticles reveals skin inflammation dynamics - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biomedical optics express Année : 2023

Real-time in vivo ROS monitoring with luminescent nanoparticles reveals skin inflammation dynamics

Résumé

Reactive oxygen species (ROS) are key regulators in numerous pathological contexts, including cancer or inflammation. Their role is complex, which justifies the need for methods enabling their quantitative and time-resolved monitoring in vivo, in the perspective to profile tissues of individual patients. However, current ROS detection methods do not provide these features. Here, we propose a new method based on the imaging of lanthanide-ion nanoparticles (GdVO 4 :Eu), whose photoluminescence is modulated by the surrounding ROS concentration. We monitored their luminescence after intradermic injection in a mouse ear submitted to an inflammation-inducing topical stimulus. Based on this approach, we quantified the ROS concentration after inflammation induction and identified a two-step kinetics of ROS production, which may be attributed to the response of resident immune cells and their further recruitment at the inflammation locus.
Fichier principal
Vignette du fichier
boe-14-10-5392.pdf (2.82 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04276786 , version 1 (09-11-2023)

Identifiants

Citer

M. Abdesselem, N. Pétri, R. Kuhner, F. Mousseau, V. Rouffiac, et al.. Real-time in vivo ROS monitoring with luminescent nanoparticles reveals skin inflammation dynamics. Biomedical optics express, 2023, 14 (10), pp.5392. ⟨10.1364/boe.501914⟩. ⟨hal-04276786⟩
20 Consultations
6 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More