5-HT1A biased agonists induce different hemodynamic responses, a pharmacological MRI study
Résumé
The pharmacological MRI (phMRI) data were acquired on a 7-Tesla Bruker Biospec MR system camera (CERMEP-Imaging Platform). The modifications of blood oxygen level dependant (BOLD) signal induced by the drugs, indirectly related to changes in neuronal activity, were measured in isoflurane-anesthetized rats. A continuous T2* echo planar imaging (EPI) sequence was used during 1 hour and a repetition time (TR) of 3 seconds. All rats were scanned under four conditions in a randomized order: a saline injection and three increasing doses of 5-HT 1A agonist (0.16-0.32-0.63 mg/kg). The animals were divided in two groups, one for each molecule (n=9 for NLX-112; n=9 for NLX-101). The intraperitoneal injection of agonist or saline was performed 15 minutes after the beginning of the phMRI session, resulting in 300 baseline scans and 900 post-injection scans. A voxel-based analysis of the data was performed at an individual level (for each scan, comparison between different time periods post-injection versus baseline), with the non-specific changes of BOLD signal in ventricles and white matter as regressors of non-interest, followed by a second-level analysis in a block design using a General Linear Model (GLM) approach (comparison between agonists conditions and saline condition) with the software Statistical Parametric Mapping (SPM12, The Wellcome Trust Center for Neuroimaging, London, UK). The statistical significance was set at p<0.001 uncorrected.
Domaines
Neurosciences
Origine : Fichiers produits par l'(les) auteur(s)
Licence : CC BY - Paternité
Licence : CC BY - Paternité