Poster De Conférence Année : 2023

Haemophilus influnezae ability to form biofilms may be linked to virulence in non-typable isolates

Résumé

Introduction Non-typeable Haemophilus Influenzae (NTHi) is responsible for invasive infections that are encountered frequently in older adults but encountered in all age groups. Biofilms represent a protective mechanism that enhances bacterial resistance to clearance. Biofilm formation by Hi has been studied in only a limited number of strains, and a repertoire of genes and bacterial surface structures have been implicated in biofilm formation and maturation. These include type IV pili over-expression, the presence of fimbriae, quorum sensing, the presence of outer membrane proteins P2 and P5, and the presence of phosphorylcholine and sialic acid in the lipooligosaccharide molecule. Aim To understand the relationship between biofilm formation and virulence. Materials and Methods We analyzed the ability of NTHi and typeable isolates to form biofilms using plate assay with crystal violet staining. WGS was used to characterize Hi according to schemes of genes involved in biofilm formation. Results Invasive NTHi isolates trend towards higher biofilm indices than invasive typeable isolates regardless of age or clinical forms. Conclusions NTHi isolates lack capsule and biofilm formation may enhance NTHi survival upon acquisition and contribute to bacterial growth and invasiveness.

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hal-04270169 , version 1 (07-11-2023)

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  • HAL Id : hal-04270169 , version 1

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Nouria Belkacem, Eva Hong, Ala-Eddine Deghmane, Muhamed-Kheir Taha. Haemophilus influnezae ability to form biofilms may be linked to virulence in non-typable isolates. 16th EMGM Congress, May 2023, Dubrovnick, Croatia. 2017. ⟨hal-04270169⟩

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