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Chapitre D'ouvrage Année : 2022

MT1 Melatonin Receptor Reconstitution in Nanodiscs

Résumé

A way to study G protein-coupled receptors in a minimal system is to reconstruct artificial membrane mimics, made of detergent and/or of lipids in which the purified receptor is maintained. In particular, it is now possible to generate lipid nanoparticles, such as nanodiscs, in which a single receptor molecule is included. Such objects offer the invaluable potential of studying an isolated receptor stabilized in a finely controlled membrane-like environment to evaluate its pharmacology, its function, and its structure at the molecular level. In this chapter, we detail the different steps from the extraction and isolation of a recombinant MT1 melatonin receptor in detergent, down to its reconstitution into nanodiscs. A G protein activation test is further described in order to exemplify how the functionality of such particles may be investigated.

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Dates et versions

hal-04266017 , version 1 (31-10-2023)

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Jean Boutin, Christel Logez, Marjorie Damian, Renaud Wagner, Jean-Louis Banères, et al.. MT1 Melatonin Receptor Reconstitution in Nanodiscs. Melatonin, 2550, Springer US, pp.171-178, 2022, Methods in Molecular Biology, ⟨10.1007/978-1-0716-2593-4_21⟩. ⟨hal-04266017⟩
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