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Pré-Publication, Document De Travail Année : 2019

Quaternary structural convergence and structural diversification of prion assemblies at the early replication stage

Résumé

Abstract Aggregation of misfolded forms from host-encoded proteins is key to the pathogenesis of a number of neurodegenerative disorders, including prion diseases, Alzheimer’s disease and Parkinson’s disease. In prion diseases, the cellular prion protein PrP C can misfold into PrP Sc and auto-organize into conformationally distinct assemblies or strains. A plethora of observations reports the existence of PrP Sc structural heterogeneity within prion strains, suggesting the emergence and coevolution of structurally distinct PrP Sc assemblies during prion replication in controlled environment. Such PrP Sc diversification processes remain poorly understood. Although central to prion host-adaptation, structural diversification of PrP Sc assemblies is also a key issue for the formation of PrP conformers involved in neuronal injury. Here, we characterized the evolution of the PrP Sc quaternary structure during prion replication in vivo and in bona fide cell-free amplification assays. Regardless of the strain studied, the early replication stage conduced to the preferential formation of small PrP Sc oligomers, thus highlighting a quaternary structural convergence phenomenon. Their evolutionary kinetics revealed the existence of a PrP C -dependent secondary templating pathway in concert with a structural rearrangement. This secondary templating pathway provides, for the first time, a mechanistic explanation for prion structural diversification during replication, a key determinant for prion adaptation on further transmission, including to other host species. The uncovered processes are also key for a better understanding of the accumulation mechanisms of other misfolded assemblies believed to propagate by a prion-like process.
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Dates et versions

hal-04264878 , version 1 (30-10-2023)

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Angélique Igel-Egalon, Florent Laferrière, Mohammed Moudjou, Mathieu Merzach, Tina Knäpple, et al.. Quaternary structural convergence and structural diversification of prion assemblies at the early replication stage. 2023. ⟨hal-04264878⟩
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