CD95L concatemers highlight different stoichiometries of CD95‐mediated apoptotic and non‐apoptotic pathways - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue European Journal of Immunology Année : 2023

CD95L concatemers highlight different stoichiometries of CD95‐mediated apoptotic and non‐apoptotic pathways

Résumé

To better understand the stoichiometry of CD95L required to trigger apoptotic and nonapoptotic signals, we generated several CD95L concatemers from dimer to hexamer conjugated via a flexible link (GGGGS) . These ligands reveal that although the hexameric structure is the best stoichiometry to trigger cell death, a dimer is sufficient to induce the apoptotic response in CD95-sensitive Jurkat cells. Interestingly, only trimeric and hexameric forms can implement a potent Ca response, suggesting that while CD95 aggregation controls the implementation of the apoptotic signal, both aggregation and conformation are required to implement the Ca pathway.
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Dates et versions

hal-04262112 , version 1 (11-01-2024)

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Eden Lebrault, Christelle Oblet, Keerthi Kurma, Nicolas Levoin, Robin Jeannet, et al.. CD95L concatemers highlight different stoichiometries of CD95‐mediated apoptotic and non‐apoptotic pathways. European Journal of Immunology, 2023, pp.2350626. ⟨10.1002/eji.202350626⟩. ⟨hal-04262112⟩
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