Transport infrastructure and services are the means to improve the well-being of all groups of the population, including women, men, children, the elderly, and people with disabilities. However, they are often incorrectly considered 'gender-neutral'. In fact, mobility is socially determined by gender roles related to reproduction, production, and community. This research investigates the cohesive relationship of urban transportation including Mobility, Gender, and Care by studying the case of HoChiMinh city (HCMC). The differences between women's and men's participation in transportation are identified and connected to the social-gender role activities. The findings show a clear relationship between urban mobility and gender roles. There is clear evidence of women's trade-off between traveling less than men and carrying more unpaid work in the family, which is more pronounced for married women who live with family. Differences in women's traffic experiences with men's reveals the disadvantage of women in traffic. The findings of this research will assist the HCMC transport sector and project executive agencies in designing gender-inclusive projects. The dissemination of this research will draw attention to the gender dimension of transport and encourage gender mainstreaming across the transport sector to promote and support gender equality and women's empowerment.