Synthesis, characterization and cytotoxicity of the Eugenia brejoensis essential oil inclusion complex with β-cyclodextrin
Résumé
This study investigated for the first time the effect of Eugenia brejoensis essential oil (EB) and its inclusion complex (IC) with β-cyclodextrin (β-CD) on Hela and J774 cell lines. Solid IC was prepared by the coprecipitation method and characterized by several analytical methods: differential scanning calorimetry (DSC), thermogravimetry analysis (TGA), X-ray diffraction (XRD), RAMAN spectroscopy and scanning electron microscopy (SEM). The loading capacity (LC) was determined. The formation constant of β-CD/caryophyllene (the main constituent of EB essential oil) as well as the ability of β-CD to retain EB and caryophyllene were evaluated by static headspace gas chromatography (SH-GC) in aqueous solution. Finally, the cytotoxicity of EB and β-CD/EB IC was determined on Hela and J774 cell lines. We clearly demonstrated the formation of an inclusion complex between EB EO and β-CD in solid state and aqueous solution. Indeed, the amount of EB included in the solid IC was equal to 4.7 μg of essential oil/mg of solid complex and the obtained formation constant between β-CD and β-(E)-caryophyllene, the main constituent of the EB, was as high as 28460 M−1. Moreover we could observe a greater selectivity of the β-CD/EB IC for the tumor lineage. Therefore, β-CD/EB IC could be a promising agent with potential application in anticancer drug development.