Multiplexed targeted analysis of polyunsaturated fatty acids and oxylipins using liquid chromatography-tandem mass spectrometry - Archive ouverte HAL
Article Dans Une Revue STAR Protocols Année : 2023

Multiplexed targeted analysis of polyunsaturated fatty acids and oxylipins using liquid chromatography-tandem mass spectrometry

Résumé

Polyunsaturated fatty acids (PUFAs) and their oxidized products (oxylipins) are important mediators in intra-and extra-cellular signaling. We describe here the simultaneous quantification of 163 PUFAs and oxylipins using liquid chromatography-mass spectrometry (LC-MS). The protocol details steps for PUFA purification from various biological materials, the conditions for LC-MS analysis, as well as quantitative approaches for data evaluation. We provide an example of PUFA quantification in animal tissue along with the bioinformatic protocol, enabling efficient inter-sample comparison and statistical analysis.

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Sciences du Vivant [q-bio]
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https://hal.science/hal-04241222

Soumis le : mardi 17 octobre 2023-11:26:47

Dernière modification le : jeudi 7 novembre 2024-16:14:03

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hal-04241222 , version 1 (17-10-2023)

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Evgenia Turtoi, Jeremy Jeudy, Gilles Valette, Christine Enjalbal, Isabelle K Vila, et al.. Multiplexed targeted analysis of polyunsaturated fatty acids and oxylipins using liquid chromatography-tandem mass spectrometry. STAR Protocols, 2023, 4, ⟨10.1016/j.xpro.2023.102226⟩. ⟨hal-04241222⟩

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CNRS FNCLCC VALDAURELLE INC-CNRS BS CHIMIE UNIV-MONTPELLIER IRCM
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