Luteolin Isolated from Juncus acutus L., a Potential Remedy for Human Coronavirus 229E - Archive ouverte HAL
Journal Articles Molecules Year : 2023

Luteolin Isolated from Juncus acutus L., a Potential Remedy for Human Coronavirus 229E

Riadh Ksouri
  • Function : Author

Abstract

The COVID-19 pandemic, caused by SARS-CoV-2, addressed the lack of specific antiviral drugs against coronaviruses. In this study, bioguided fractionation performed on both ethyl acetate and aqueous sub-extracts of Juncus acutus stems led to identifying luteolin as a highly active antiviral molecule against human coronavirus HCoV-229E. The apolar sub-extract (CH2Cl2) containing phenanthrene derivatives did not show antiviral activity against this coronavirus. Infection tests on Huh-7 cells, expressing or not the cellular protease TMPRSS2, using luciferase reporter virus HCoV-229E-Luc showed that luteolin exhibited a dose-dependent inhibition of infection. Respective IC50 values of 1.77 µM and 1.95 µM were determined. Under its glycosylated form (luteolin-7-O-glucoside), luteolin was inactive against HCoV-229E. Time of addition assay showed that utmost anti-HCoV-229E activity of luteolin was achieved when added at the post-inoculation step, indicating that luteolin acts as an inhibitor of the replication step of HCoV-229E. Unfortunately, no obvious antiviral activity for luteolin was found against SARS-CoV-2 and MERS-CoV in this study. In conclusion, luteolin isolated from Juncus acutus is a new inhibitor of alphacoronavirus HCoV-229E.
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Dates and versions

hal-04237724 , version 1 (11-10-2023)

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Asma Hakem, Lowiese Desmarets, Ramla Sahli, Rawen Ben Malek, Charline Camuzet, et al.. Luteolin Isolated from Juncus acutus L., a Potential Remedy for Human Coronavirus 229E. Molecules, 2023, 28 (11), pp.4263. ⟨10.3390/molecules28114263⟩. ⟨hal-04237724⟩
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