One step bio-guided isolation of secondary metabolites from the endophytic fungus Penicillium crustosum using high-resolution semipreparative HPLC
Résumé
An endophytic fungal strain Penicillium crustosum was isolated from the seagrass Posidonia oceanica and investigated to identify its antimicrobial constituents and characterize its metabolome composition. The ethyl acetate extract of this fungus exhibited antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) as well as an anti-quorum sensing effect against Pseudomonas aeruginosa. The crude extract was profiled by UHPLC-HRMS/MS and the dereplication was assisted by feature-based molecular networking. As a result, more than twenty compounds were annotated in this fungus. To rapidly identify the active compounds, the enriched extract was fractionated by semi-preparative HPLC-UV applying a chromatographic gradient transfer and dry load sample introduction to maximise resolution. The collected fractions were profiled by 1 H-NMR and UHPLC-HRMS. This method led to the unambiguous identification of eight known secondary metabolites as well as the determination of their bioactivity using a single purification step.
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Licence : CC BY - Paternité