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Article Dans Une Revue Molecules Année : 2023

Modification of a Marine Pine Kraft Lignin Sample by Enzymatic Treatment with a Pycnoporus cinnabarinus Laccase

Florian Pion
Elise Martin
  • Fonction : Auteur
Annick Doan
  • Fonction : Auteur
  • PersonId : 1290179
Anne Lomascolo
Craig B Faulds
Stéphanie Baumberger
Hélène de Baynast
  • Fonction : Auteur
  • PersonId : 1290181
Fabrice Audonnet
Paul-Henri Ducrot
Philippe Michaud
  • Fonction : Auteur
  • PersonId : 1024654

Résumé

Here, we report work on developing an enzymatic process to improve the functionalities of industrial lignin. A kraft lignin sample prepared from marine pine was treated with the high-redoxpotential laccase from the basidiomycete fungus Pycnoporus cinnabarinus at three different concentrations and pH conditions, and with and without the chemical mediator 1-hydroxybenzotriazole (HBT). Laccase activity was tested in the presence and absence of kraft lignin. The optimum pH of PciLac was initially 4.0 in the presence and absence of lignin, but at incubation times over 6 h, higher activities were found at pH 4.5 in the presence of lignin. Structural changes in lignin were investigated by Fourier-transform infrared spectroscopy (FTIR) with differential scanning calorimetry (DSC), and solvent-extractable fractions were analyzed using high-performance size-exclusion chromatography (HPSEC) and gas chromatography-mass spectrometry (GC-MS). The FTIR spectral data were analyzed with two successive multivariate series using principal component analysis (PCA) and ANOVA statistical analysis to identify the best conditions for the largest range of chemical modifications. DSC combined with modulated DSC (MDSC) revealed that the greatest effect on glass transition temperature (Tg) was obtained at 130 U g cm −1 and pH 4.5, with the laccase alone or combined with HBT. HPSEC data suggested that the laccase treatments led to concomitant phenomena of oligomerization and depolymerization, and GC-MS revealed that the reactivity of the extractable phenolic monomers depended on the conditions tested. This study demonstrates that P. cinnabarinus laccase can be used to modify marine pine kraft lignin, and that the set of analytical methods implemented here provides a valuable tool for screening enzymatic treatment conditions.
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hal-04228332 , version 1 (04-10-2023)

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Sona Malric-Garajova, Florian Fortuna, Florian Pion, Elise Martin, Adithya Raveendran Thottathil, et al.. Modification of a Marine Pine Kraft Lignin Sample by Enzymatic Treatment with a Pycnoporus cinnabarinus Laccase. Molecules, 2023, 28 (12), pp.4873. ⟨10.3390/molecules28124873⟩. ⟨hal-04228332⟩
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