Performance evaluation of a PCR panel (FilmArray® Pneumonia Plus) for detection of respiratory bacterial pathogens in respiratory specimens: A systematic review and meta-analysis - Archive ouverte HAL
Article Dans Une Revue Anaesthesia Critical Care & Pain Medicine Année : 2023

Performance evaluation of a PCR panel (FilmArray® Pneumonia Plus) for detection of respiratory bacterial pathogens in respiratory specimens: A systematic review and meta-analysis

Résumé

Background: Accuracy and timing of antibiotic therapy remain a challenge for lower respiratory tract infections. New molecular techniques using Multiplex Polymerase Chain Reaction, including the FilmArray® Pneumonia Plus Panel [FAPP], have been developed to address this. The aim of this study is to evaluate the FAPP diagnostic performance for the detection of the 15 typical bacteria of the panel from respiratory samples in a meta-analysis from a systematic review. Methods: We searched PubMed and EMBASE from January 1, 2010, to December 31, 2022, and selected any study on the FAPP diagnostic performance on respiratory samples compared to the reference standard, bacterial culture. The main outcome was the overall diagnostic accuracy with sensitivity and specificity. We calculated the log Diagnostic Odds Ratio and analyzed performance for separate bacteria, antimicrobial resistance genes, and according to the sample type. We also reported the FAPP turnaround time and the out-of-panel bacteria number and species. This study is registered with PROSPERO (CRD42021226280). Results: From 10 317 records, we identified 30 studies including 8 968 samples. Twenty-one were related to intensive care. The overall sensitivity and specificity were 94% [95% Confidence Interval (CI) 91–95] and 98% [95%CI 97–98], respectively. The log Diagnostic Odds Ratio was 6.35 [95%CI 6.05–6.65]. 9.3% [95%CI 9.2–9.5] of bacteria detected in culture were not included in the FAPP panel. Conclusion: This systematic review reporting the FAPP evaluation revealed a high accuracy. This test may represent an adjunct tool for pulmonary bacterial infection diagnostic and antimicrobial stewardship. Further evidence is needed to assess the impact on clinical outcome. © 2023 Société française d'anesthésie et de réanimation (Sfar)
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Dates et versions

hal-04226750 , version 1 (03-10-2023)

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Anne-Clotilde Moy, Antoine Kimmoun, Thomas Merkling, Béatrice Berçot, François Caméléna, et al.. Performance evaluation of a PCR panel (FilmArray® Pneumonia Plus) for detection of respiratory bacterial pathogens in respiratory specimens: A systematic review and meta-analysis. Anaesthesia Critical Care & Pain Medicine, 2023, 42 (6), pp.101300. ⟨10.1016/j.accpm.2023.101300⟩. ⟨hal-04226750⟩
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