3D‐Printed Osteoinductive Polymeric Scaffolds with Optimized Architecture to Repair a Sheep Metatarsal Critical‐Size Bone Defect - Archive ouverte HAL Access content directly
Journal Articles Advanced Healthcare Materials Year : 2023

3D‐Printed Osteoinductive Polymeric Scaffolds with Optimized Architecture to Repair a Sheep Metatarsal Critical‐Size Bone Defect

Claire Deroy
  • Function : Author
Samantha Roques
  • Function : Author
Laurence Bordenave
  • Function : Author
Marlène Durand
  • Function : Author
Catherine Picart

Abstract

Abstract The reconstruction of critical‐size bone defects in long bones remains a challenge for clinicians. A new osteoinductive medical device is developed here for long bone repair by combining a 3D‐printed architectured cylindrical scaffold made of clinical‐grade polylactic acid (PLA) with a polyelectrolyte film coating delivering the osteogenic bone morphogenetic protein 2 (BMP‐2). This film‐coated scaffold is used to repair a sheep metatarsal 25‐mm long critical‐size bone defect. In vitro and in vivo biocompatibility of the film‐coated PLA material is proved according to ISO standards. Scaffold geometry is found to influence BMP‐2 incorporation. Bone regeneration is followed using X‐ray scans, µCT scans, and histology. It is shown that scaffold internal geometry, notably pore shape, influenced bone regeneration, which is homogenous longitudinally. Scaffolds with cubic pores of ≈870 µm and a low BMP‐2 dose of ≈120 µg cm −3 induce the best bone regeneration without any adverse effects. The visual score given by clinicians during animal follow‐up is found to be an easy way to predict bone regeneration. This work opens perspectives for a clinical application in personalized bone regeneration.
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Origin : Publication funded by an institution

Dates and versions

hal-04225190 , version 1 (02-10-2023)

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Charlotte Garot, Sarah Schoffit, Cécile Monfoulet, Paul Machillot, Claire Deroy, et al.. 3D‐Printed Osteoinductive Polymeric Scaffolds with Optimized Architecture to Repair a Sheep Metatarsal Critical‐Size Bone Defect. Advanced Healthcare Materials, 2023, ⟨10.1002/adhm.202301692⟩. ⟨hal-04225190⟩
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