Tuning of the Chl$_{D1}$ and Chl$_{D2}$ properties in photosystem II by site-directed mutagenesis of neighbouring amino acids - Archive ouverte HAL Access content directly
Journal Articles Biochimica biophysica acta (BBA) - Bioenergetics Year : 2024

Tuning of the Chl$_{D1}$ and Chl$_{D2}$ properties in photosystem II by site-directed mutagenesis of neighbouring amino acids

Abstract

Photosystem II is the water/plastoquinone photo-oxidoreductase of photosynthesis. The photochemistry and catalysis occur in a quasi-symmetrical heterodimer, D1D2, that evolved from a homodimeric ancestor. Here, we studied site-directed mutants in PSII from the thermophilic cyanobacterium $Thermosynechoccocus\ elongatus$, focusing on the primary electron donor chlorophyll a in D1, Chl$_{D1}$ , and on its symmetrical counterpart in D2, Chl$_{D2}$ , which does not play a direct photochemical role. The main conserved amino acid specific to Chl $_{D1}$ is D1/ T179, which H-bonds the water ligand to its Mg$^{2+}$ , while its counterpart near Chl$_{D2}$ is the non-H-bonding D2/ I178. The symmetrical-swapped mutants, D1/T179I and D2/I178T, and a second Chl$_{D2}$ mutant, D2/I178H, were studied. The D1 mutations affected the 686 nm absorption attributed to Chl $_{D1}$ , while the D2 mutations affected a 663 nm feature, tentatively attributed to Chl$_{D2}$. The mutations had little effect on enzyme activity and forward electron transfer, reflecting the robustness of the overall enzyme function. In contrast, the mutations significantly affected photodamage and protective mechanisms, reflecting the importance of redox tuning in these processes. In D1/T179I, the radical pair recombination triplet on Chl$_{D1}$ was shared onto a pheophytin, presumably Phe D1 and the detection of $^3$Phe$_{D1}$ supports the proposed mechanism for the anomalously short lifetime of $^3$Chl$_{D1}$ ; e.g. electron transfer quenching by Q A − of $^3$Phe$_{D1}$ after triplet transfer from $^3$Chl$_{D1}$. In D2/I178T, a charge separation could occur between Chl$_{D2}$ and Phe$_{D2}$ , a reaction that is thought to occur in ancestral precursors of PSII. These mutants help understand the evolution of asymmetry in PSII.
Embargoed file
Embargoed file
0 5 8
Year Month Jours
Avant la publication
Thursday, September 26, 2024
Embargoed file
Thursday, September 26, 2024
Please log in to request access to the document

Dates and versions

hal-04217714 , version 1 (26-09-2023)

Identifiers

Cite

Miwa Sugiura, Masaya Kimura, Naohiro Shimamoto, Yuki Takegawa, Makoto Nakamura, et al.. Tuning of the Chl$_{D1}$ and Chl$_{D2}$ properties in photosystem II by site-directed mutagenesis of neighbouring amino acids. Biochimica biophysica acta (BBA) - Bioenergetics, 2024, 1865, pp.149013. ⟨10.1016/j.bbabio.2023.149013⟩. ⟨hal-04217714⟩
64 View
1 Download

Altmetric

Share

Gmail Facebook X LinkedIn More