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            <title xml:lang="en">Development of a miniaturized solid phase extraction of enzymatic oxylipins for a miniaturized analysis on a UHPLC-ESI-MS/MS Agilent</title>
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                <term xml:lang="en">electrospray ionization</term>
                <term xml:lang="en">solid phase extraction</term>
                <term xml:lang="en">analytical chemistry</term>
                <term xml:lang="en">sample preparation</term>
                <term xml:lang="en">low resolution</term>
                <term xml:lang="en">mass spectrometry</term>
                <term xml:lang="en">liquid chromatography</term>
                <term xml:lang="en">enzymatic oxylipins</term>
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              <p>Oxylipins are important lipid mediators from the metabolism of polyunsaturated fatty acids such as archidonic acid, linoleic acid, alpha-linoleic acid, eicosapentanoic acid and docosahexanoic acid through enzymatic reactions. In mammals, oxylipins have an essential role in many physiological processes such as the regulation of the cardiac, renal or vascular system. These compounds are also involved in the development of chronic diseases when their biosynthetic pathways are disrupted.Due to their very low concentration, a specific sample preparation and a high sensitive analytical method are required to quantify them in biological samples. They are therefore analysed with a new highly sensitive analytical method on a micro-liquid chromatography coupled to tandem mass spectrometry with a micro-electrospray ionisation source (NexeraX2-LCMS8060, Shimadzu). Research is progressing towards smaller quantities of matrices, for example a tumour biopsy does not exceed 2mg. Thus, one of the challenge is to quantify oxylipins in a very small amount of samples. In this context, we have developed a miniaturized solid phase extraction (SPE) protocol using 2 mg SPE plate (OasisHLB, Waters). This miniaturization makes it possible to gain in sensitivity by reducing the loss of analyte. The sample is enriched by eliminating drying and recovery steps. This protocol also allows limited and small sample volumes to be processed while still having linear, accurate and precise results for all analytes. In addition, a miniaturised protocol makes possible as well to move towards an eco-responsible approach by reducing the carbon cost of the sample preparation and of the analysis.</p>
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