HPLC-DAD optimization of quantification of vescalagin, gallic and ellagic acid in chestnut tannins - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Heliyon Année : 2023

HPLC-DAD optimization of quantification of vescalagin, gallic and ellagic acid in chestnut tannins

Philippe Jacolot
Luc Goethals
  • Fonction : Auteur
  • PersonId : 1147356
  • IdRef : 263403955
Nicolas Barbezier
Pauline M Anton

Résumé

The quantification of hydrolysable polyphenols such as gallic, ellagic acid and vescalagin by HPLC-DAD is classically run after methanol extraction as a reference solvent. Water extraction is usually discarded because of a lower obtention of total polyphenol content compared to methanol extraction. In our study, methanol was compared to water extraction in both the total polyphenol content method and the HPLC-DAD analysis. Total polyphenol content in water extraction was lower than in methanol extraction, but water extraction gave better results on HPLC-DAD. In conclusion, total polyphenol content cannot be used as reference to choose the solvent of extraction to quantify some polyphenols by HPLC-DAD because of the specific properties of each polyphenol. Indeed, recovery results obtained on hydrolysable polyphenols with water extraction were better and with a lower variability than following methanol extraction.
Fichier principal
Vignette du fichier
PIIS2405844023062011.pdf (907.27 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04213639 , version 1 (21-09-2023)

Identifiants

Citer

Charline Richard-Dazeur, Philippe Jacolot, Céline Niquet-Léridon, Luc Goethals, Nicolas Barbezier, et al.. HPLC-DAD optimization of quantification of vescalagin, gallic and ellagic acid in chestnut tannins. Heliyon, 2023, 9 (8), pp.e18993. ⟨10.1016/j.heliyon.2023.e18993⟩. ⟨hal-04213639⟩
20 Consultations
20 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More