Article Dans Une Revue Journal of Cell Science Année : 2023

Glucose oxidation drives trunk neural crest cell development and fate

Glucose oxidation drives trunk neural crest cell development and fate

Redouane Fodil
Sophie Féréol
Alwyn Dady
Marine Depp
Frederic Relaix
Roberto Motterlini
Jean-Loup Duband
Sylvie Dufour

Résumé

Bioenergetic metabolism is a key regulator of cellular function and signaling, but how it can instruct the behavior of cells and their fate during embryonic development remains largely unknown. Here, we investigated the role of glucose metabolism in the development of avian trunk neural crest cells (NCCs), a migratory stem cell population of the vertebrate embryo. We uncovered that trunk NCCs display glucose oxidation as a prominent metabolic phenotype, in contrast to what is seen for cranial NCCs, which instead rely on aerobic glycolysis. In addition, only one pathway downstream of glucose uptake is not sufficient for trunk NCC development. Indeed, glycolysis, mitochondrial respiration and the pentose phosphate pathway are all mobilized and integrated for the coordinated execution of diverse cellular programs, epithelial-to-mesenchymal transition, adhesion, locomotion, proliferation and differentiation, through regulation of specific gene expression. In the absence of glucose, the OXPHOS pathway fueled by pyruvate failed to promote trunk NCC adaptation to environmental stiffness, stemness maintenance and fate-decision making. These findings highlight the need for trunk NCCs to make the most of the glucose pathway potential to meet the high metabolic demands appropriate for their development.

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Dates et versions

hal-04208813 , version 1 (15-09-2023)

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Nioosha Nekooie Marnany, Redouane Fodil, Sophie Féréol, Alwyn Dady, Marine Depp, et al.. Glucose oxidation drives trunk neural crest cell development and fate. Journal of Cell Science, 2023, 136 (16), ⟨10.1242/jcs.260607⟩. ⟨hal-04208813⟩
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