Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus -related and V. aestuarianus strains from bacterial cultures - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Microbiological Methods Année : 2017

Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus -related and V. aestuarianus strains from bacterial cultures

Résumé

To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies.

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hal-04201905 , version 1 (11-09-2023)

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Denis Saulnier, Sophie de Decker, Delphine Tourbiez, Marie-Agnes Travers. Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus -related and V. aestuarianus strains from bacterial cultures. Journal of Microbiological Methods, 2017, 140, pp.67-69. ⟨10.1016/j.mimet.2017.07.002⟩. ⟨hal-04201905⟩

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