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Poster De Conférence Année : 2023

Contribution of plasma proteins to the phenotypic signature of feed efficiency in Charolais bulls

Résumé

Improving the efficiency of feed utilization by reducing the amount of feed intake while maintaining the same level of production has significant economic and environmental impacts in beef cattle. One major challenge is to easily phenotype this multifactorial animal trait for the purpose of genetic selection and precision feeding. Biomarkers and proxies of feed efficiency (FE) may contribute to this challenge. In order to identify phenotypic signatures that discriminate FE groups, we characterized 17 Charolais young bulls fed grass silage diets on their FE parameters (Residual Feed Intake: RFI, and feed conversion efficiency: FCE), carcass composition, some targeted blood parameters (hormones: GH, IGF, insulin, adiponectin, leptin, FT4; metabolites: glucose, NEFA, alpha amino nitrogen, BOH butyrate, urea), and plasma proteome.Labelled-free shotgun proteomics enabled identifying 229 plasma proteins (with 2 peptides, 1% FDR). Univariate, multivariate, and a multiblock sPLSDA analyses were applied to obtain a signature combining omics and carcass traits that discriminates the groups of FE animals. For that, we integrated our multiple datasets while explaining their relationship with FE variables (DIABLO method, mixOmics package). For RFI, the first latent variable of plasma protein abundances was strongly correlated with the one of carcass traits (r= 0.63) and with the one of blood parameters (r=0.68) and enabled a clear discrimination of the groups. The key variables that drove such discrimination were identified as 25 proteins together with 3 carcass parameters and 8 hormones and metabolites. Inefficient bulls (with positive RFI) were characterized by 1) bigger bladder and heart; 2) higher GH, adiponectin and FT4 levels, as well as lower insulin and IGF1 levels, higher alpha amino nitrogen and NEFA levels; 3) higher abundance in 20 plasma proteins (incl. ITIH3, IPSP, ASPN and PSA6) and lower abundance in 5 plasma proteins (incl. MBL2, ANGPTL3, CO3).
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Dates et versions

hal-04196518 , version 1 (11-09-2023)

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I Cassar-Malek, A Imbert, A Delavaud, H Sauerwein, R Bruckmaier, et al.. Contribution of plasma proteins to the phenotypic signature of feed efficiency in Charolais bulls. Scientific Committee. 74th Annual Meeting of the European Federation of Animal Science, Aug 2023, Lyon, France. Wageningen Academic Publishers, Book of Abstracts of the 74thAnnual Meeting of the European Federation of Animal Science, 29, pp.490, 2023, ⟨10.3920/978-90-8686-936-7⟩. ⟨hal-04196518⟩

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