Enzyme-immobilized hierarchically porous covalent organic framework biocomposite for catalytic degradation of broad-range emerging pollutants in water - Archive ouverte HAL Access content directly
Journal Articles Journal of Hazardous Materials Year : 2023

Enzyme-immobilized hierarchically porous covalent organic framework biocomposite for catalytic degradation of broad-range emerging pollutants in water

Nada Elmerhi
  • Function : Author
Khadega Al-Maqdi
  • Function : Author
Khawlah Athamneh
  • Function : Author
Abdul Khayum Mohammed
  • Function : Author
Tina Skorjanc
  • Function : Author
Felipe Gándara
  • Function : Author
Jesus Raya
  • Function : Author
Olivier Siri
Ali Trabolsi
  • Function : Author
Iltaf Shah
  • Function : Author
Dinesh Shetty
Syed Salman Ashraf
  • Function : Author

Abstract

Efficient enzyme immobilization is crucial for the successful commercialization of large-scale enzymatic water treatment. However, issues such as lack of high enzyme loading coupled with enzyme leaching present challenges for the widespread adoption of immobilized enzyme systems. The present study describes the development and bioremediation application of an enzyme biocomposite employing a cationic macrocycle-based covalent organic framework (COF) with hierarchical porosity for the immobilization of horseradish peroxidase (HRP). The intrinsic hierarchical porous features of the azacalix[4]arene-based COF (ACA-COF) allowed for a maximum HRP loading capacity of 0.76 mg/mg COF with low enzyme leaching (<5.0 %). The biocomposite, HRP@ACA-COF, exhibited exceptional thermal stability (∼200 % higher relative activity than the free enzyme), and maintained ∼60 % enzyme activity after five cycles. LCMSMS analyses confirmed that the HRP@ACA-COF system was able to achieve > 99 % degradation of seven diverse types of emerging pollutants (2-mercaptobenzothiazole, paracetamol, caffeic acid, methylparaben, furosemide, sulfamethoxazole, and salicylic acid)in under an hour. The described enzyme-COF system offers promise for efficient wastewater bioremediation applications.
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Dates and versions

hal-04183683 , version 1 (20-08-2023)

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Nada Elmerhi, Khadega Al-Maqdi, Khawlah Athamneh, Abdul Khayum Mohammed, Tina Skorjanc, et al.. Enzyme-immobilized hierarchically porous covalent organic framework biocomposite for catalytic degradation of broad-range emerging pollutants in water. Journal of Hazardous Materials, 2023, 459, pp.132261. ⟨10.1016/j.jhazmat.2023.132261⟩. ⟨hal-04183683⟩
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