Effects of bisphenols on human granulosa cells and cocktail effect
Effets des bisphénols sur les cellules de granulosa humaines et effet cocktail
Résumé
Bisphenol (BP) structural analogues of BPA are widely used. Previous studies showed similar effects of BPA and BPS on reproduction in several species including human. We hypothesised that the similar effects of several bisphenols (BPs) could accumulate in granulosa cells (GCs) and affects steroidogenesis. This study investigated the effects of seven BP analogues and their equimolar cocktail on human granulosa cells (hGC) and assessed BPA, BPS, BPF and BPAF level exposures in the follicular fluid of 277 women undergoing Assisted Reproductive Technology. The hGCs were recovered after women oocyte punctures and treated with the seven BP analogues (BPS, BPA, BPAF, BPF, BPAP, BPE and BPB) or their equimolarcocktail of 7 × 1.43 or 7 × 7.14 μM for each of the seven BPs, the sum of BPs reaching10 (”∑BPs 10 μM”), or 50 μM (”∑BPs 50 μM”), respectively. Oestradiol and progesterone secretion, cell proliferation and viability were investigated. Progesterone secretion was decreased by 6 BPs 10 μM and the cocktail “∑BPs 10 μM”, ( 17.8 to 41.3%) and by all seven BPs 50μM and “∑BPs 50 μM” ( 21.8 to 84.2%). Oestradiol secretion was decreased only by 50 μM BPAF and BPAP ( 37.8% and 44%, respectively). Cellular proliferation was decreased after treatment with 50 μM BPAF ( 32.2%), BPAP ( 29%), BPB ( 24%) and the equimolar cocktail “∑BPs 50 μM” ( 33.1%). At least one BP was detected in 64 of 277 (23.1%) women follicular fluids. Similar effects of the seven BPs or their cocktail were observed on progesterone secretion and/or on cell proliferation, suggesting cumulative effects of BPs.
Domaines
Toxicologie et chaîne alimentaireOrigine | Fichiers produits par l'(les) auteur(s) |
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