pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
Résumé
Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS emm3 genotype.
| Origine | Fichiers éditeurs autorisés sur une archive ouverte |
|---|---|
| Licence |
