Fabrication of plasmonic metasurfaces for fluorescence nanoscopy
Résumé
Single-molecule fluorescence localization microscopy has become an indispensable technique of imaging with resolution better than 10 nanometers. However, the light emission of fluorophores is not easy to control because of photobleaching. In the presence of nanoantennas, the photobleaching lifetime, blinking rate and overall photoluminescence intensity can be strongly increased due to the Purcell effect.
We fabricated arrays of plasmonic nanoantennas organized as cyclic group metasurfaces coated with a fluorescent film for super-resolution imaging. Following a systematic study of fluorescence engineering using metamaterial-assisted localization microscopy (MALM), we show that improving the photostability of fluorophores and increasing the photoluminescence intensity in MALM enables
imaging resolution with a localization accuracy of 0.9 nm.