<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-04151647</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-23T13:59:04+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">On demand utilization of phosphoribosyl pyrophosphate by downstream anabolic pathways</title>
            <author role="aut">
              <persName>
                <forename type="first">Benoît</forename>
                <surname>Pinson</surname>
              </persName>
              <email type="md5">97394c7de753e60658a71b98cc17158d</email>
              <email type="domain">ibgc.cnrs.fr</email>
              <idno type="idhal" notation="string">benoit-pinson</idno>
              <idno type="idhal" notation="numeric">178827</idno>
              <idno type="halauthorid" notation="string">1973-178827</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-2936-9058</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?hl=en&amp;user=Crv-aNoAAAAJ</idno>
              <idno type="IDREF">https://www.idref.fr/123225531</idno>
              <affiliation ref="#struct-588"/>
              <affiliation ref="#struct-1058635"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Michel</forename>
                <surname>Moenner</surname>
              </persName>
              <idno type="idhal" notation="numeric">1267742</idno>
              <idno type="halauthorid" notation="string">618592-1267742</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-2249-4182</idno>
              <affiliation ref="#struct-588"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Christelle</forename>
                <surname>Saint-Marc</surname>
              </persName>
              <idno type="idhal" notation="numeric">797940</idno>
              <idno type="halauthorid" notation="string">1697702-797940</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-9537-1848</idno>
              <affiliation ref="#struct-588"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Alexandra</forename>
                <surname>Granger-Farbos</surname>
              </persName>
              <idno type="idhal" notation="numeric">1267743</idno>
              <idno type="halauthorid" notation="string">1724431-1267743</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-0374-2064</idno>
              <affiliation ref="#struct-1058635"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Bertrand</forename>
                <surname>Daignan-Fornier</surname>
              </persName>
              <email type="md5">234b8672e7f5baf849d849c9bd5a26a1</email>
              <email type="domain">ibgc.cnrs.fr</email>
              <idno type="idhal" notation="string">bertrand-daignan-fornier-de-lachaux</idno>
              <idno type="idhal" notation="numeric">738469</idno>
              <idno type="halauthorid" notation="string">1974-738469</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-2352-9700</idno>
              <idno type="IDREF">https://www.idref.fr/058592334</idno>
              <affiliation ref="#struct-588"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Benoît</forename>
                <surname>PINSON</surname>
              </persName>
              <email type="md5">97394c7de753e60658a71b98cc17158d</email>
              <email type="domain">ibgc.cnrs.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2023-07-05 09:08:48</date>
              <date type="whenModified">2025-10-23 17:54:36</date>
              <date type="whenReleased">2023-07-05 13:25:46</date>
              <date type="whenProduced">2023</date>
              <date type="whenEndEmbargoed">2023-07-05</date>
              <ref type="file" target="https://hal.science/hal-04151647v1/document">
                <date notBefore="2023-07-05"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://hal.science/hal-04151647v1/file/Manuscript%20Pinson%20et%20al.pdf" id="file-4151647-3616087">
                <date notBefore="2023-07-05"/>
              </ref>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="79382">
                <persName>
                  <forename>Benoît</forename>
                  <surname>PINSON</surname>
                </persName>
                <email type="md5">97394c7de753e60658a71b98cc17158d</email>
                <email type="domain">ibgc.cnrs.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-04151647</idno>
            <idno type="halUri">https://hal.science/hal-04151647</idno>
            <idno type="halBibtex">pinson:hal-04151647</idno>
            <idno type="halRefHtml">&lt;i&gt;Journal of Biological Chemistry&lt;/i&gt;, In press, &lt;a target="_blank" href="https://dx.doi.org/10.1016/j.jbc.2023.105011"&gt;&amp;#x27E8;10.1016/j.jbc.2023.105011&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Journal of Biological Chemistry, In press, &amp;#x27E8;10.1016/j.jbc.2023.105011&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-4151647-3616087"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="INSERM">INSERM - Institut national de la santé et de la recherche médicale</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UMR5095">Institut de Biochimie et Génétique Cellulaires</idno>
            <idno type="stamp" n="UNIVERSITE-BORDEAUX">Université de Bordeaux</idno>
            <idno type="stamp" n="TBMCORE">TBM-Core </idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">On demand utilization of phosphoribosyl pyrophosphate by downstream anabolic pathways</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Benoît</forename>
                    <surname>Pinson</surname>
                  </persName>
                  <email type="md5">97394c7de753e60658a71b98cc17158d</email>
                  <email type="domain">ibgc.cnrs.fr</email>
                  <idno type="idhal" notation="string">benoit-pinson</idno>
                  <idno type="idhal" notation="numeric">178827</idno>
                  <idno type="halauthorid" notation="string">1973-178827</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-2936-9058</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?hl=en&amp;user=Crv-aNoAAAAJ</idno>
                  <idno type="IDREF">https://www.idref.fr/123225531</idno>
                  <affiliation ref="#struct-588"/>
                  <affiliation ref="#struct-1058635"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Michel</forename>
                    <surname>Moenner</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">1267742</idno>
                  <idno type="halauthorid" notation="string">618592-1267742</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-2249-4182</idno>
                  <affiliation ref="#struct-588"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Christelle</forename>
                    <surname>Saint-Marc</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">797940</idno>
                  <idno type="halauthorid" notation="string">1697702-797940</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-9537-1848</idno>
                  <affiliation ref="#struct-588"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Alexandra</forename>
                    <surname>Granger-Farbos</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">1267743</idno>
                  <idno type="halauthorid" notation="string">1724431-1267743</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-0374-2064</idno>
                  <affiliation ref="#struct-1058635"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Bertrand</forename>
                    <surname>Daignan-Fornier</surname>
                  </persName>
                  <email type="md5">234b8672e7f5baf849d849c9bd5a26a1</email>
                  <email type="domain">ibgc.cnrs.fr</email>
                  <idno type="idhal" notation="string">bertrand-daignan-fornier-de-lachaux</idno>
                  <idno type="idhal" notation="numeric">738469</idno>
                  <idno type="halauthorid" notation="string">1974-738469</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-2352-9700</idno>
                  <idno type="IDREF">https://www.idref.fr/058592334</idno>
                  <affiliation ref="#struct-588"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">5882</idno>
                <idno type="issn">0021-9258</idno>
                <idno type="eissn">1083-351X</idno>
                <title level="j">Journal of Biological Chemistry</title>
                <imprint>
                  <publisher>American Society for Biochemistry and Molecular Biology</publisher>
                  <date type="datePub" subtype="inPress">2023</date>
                  <date type="dateEpub">2023-07-04</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1016/j.jbc.2023.105011</idno>
              <ref type="publisher">https://www.jbc.org/article/S0021-9258(23)02039-2/fulltext</ref>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">Pentose phosphate pathway</term>
                <term xml:lang="en">nucleotide biosynthesis</term>
                <term xml:lang="en">phosphoribosyl pyrophosphate</term>
                <term xml:lang="en">yeast</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv.bbm.bc">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>The pentose phosphate pathway (PPP) is critical for anabolism and biomass production. Here we show that the essential function of PPP in yeast is the synthesis of phosphoribosyl pyrophosphate (PRPP) catalyzed by PRPP-synthetase. Using combinations of yeast mutants, we found that a mildly decreased synthesis of PRPP affects biomass production, resulting in reduced cell size, while a more severe decrease ends up affecting yeast doubling time. We establish that it is PRPP itself that is limiting in invalid PRPP-synthetase mutants and that the resulting metabolic and growth defect can be bypassed by proper supplementation of the medium with ribose-containing precursors or by the expression of bacterial or human PRPPsynthetase. In addition, using documented pathologic human hyperactive forms of PRPPsynthetase, we show that intracellular PRPP as well as its derived products can be increased in both human and yeast cells and we describe the ensuing metabolic and physiological consequences. Finally, we found that PRPP consumption appears to take place "on demand" by the various PRPP-utilizing pathways, as shown by blocking or increasing the flux in specific PRPP-consuming metabolic routes. Overall, our work reveals important similarity between human and yeast for both synthesis and consumption of PRPP.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-588" status="VALID">
          <idno type="IdRef">185481035</idno>
          <idno type="ISNI">0000000417952841</idno>
          <idno type="RNSR">199911781P</idno>
          <idno type="ROR">https://ror.org/048xwe611</idno>
          <orgName>Institut de biochimie et génétique cellulaires</orgName>
          <orgName type="acronym">IBGC</orgName>
          <date type="start">1994-01-01</date>
          <desc>
            <address>
              <addrLine>1 Rue Camille Saint-Saens 33077 BORDEAUX CEDEX</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.ibgc.cnrs.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-259761" type="direct"/>
            <relation name="UMR5095 / UPR9026" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-1058635" status="VALID">
          <orgName>TBM-Core [Bordeaux]</orgName>
          <orgName type="acronym">UAR3427 - INSERM US005</orgName>
          <date type="start">2011-01-01</date>
          <desc>
            <address>
              <addrLine>Université Bordeaux Segalen B1A 146 rue Léo Saignat 33076 BORDEAUX CEDEX - France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.tbmcore.cnrs.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-259761" type="direct"/>
            <relation name="US 005" active="#struct-303623" type="direct"/>
            <relation name="UAR3427 / UMS3427" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-259761" status="VALID">
          <idno type="IdRef">175206562</idno>
          <idno type="ROR">https://ror.org/057qpr032</idno>
          <orgName>Université de Bordeaux</orgName>
          <orgName type="acronym">UB</orgName>
          <desc>
            <address>
              <addrLine>35, place Pey Berland - 33076 Bordeaux</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.u-bordeaux.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-303623" status="VALID">
          <idno type="IdRef">026388278</idno>
          <idno type="ROR">https://ror.org/02vjkv261</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>
            <address>
              <addrLine>101, rue de Tolbiac, 75013 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>