Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae) - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2019

Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae)

Sudip Some
  • Fonction : Auteur
Onur Bulut
  • Fonction : Auteur
Kinkar Biswas
  • Fonction : Auteur
Anupam Roy
  • Fonction : Auteur
Ipsita Kumar Sen
  • Fonction : Auteur
Amitava Mandal
  • Fonction : Auteur
Octavio Franco
  • Fonction : Auteur
Kartik Neog
  • Fonction : Auteur
Sayantan Pradhan
  • Fonction : Auteur
Subhadeep Dutta
  • Fonction : Auteur
Debjoy Bhattacharjya
  • Fonction : Auteur
Soumen Saha
  • Fonction : Auteur
Pradeep das Mohapatra
  • Fonction : Auteur
Anil Bhuimali
  • Fonction : Auteur
B. Unni
  • Fonction : Auteur
Ahmet Kati
  • Fonction : Auteur
M. Deniz Yilmaz
  • Fonction : Auteur
Ismail Ocsoy
  • Fonction : Auteur

Résumé

AbstractHerein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance. The average diameter (~54 nm) of AgNPs was measured from HR-TEM analysis. EDX spectra also supported the formation of AgNPs, and negative zeta potential value (−14 mV) suggested its stability. Moreover, a shift in the carbonyl stretching (from 1639 cm−1 to 1630 cm−1) was noted in the FT-IR spectra of leaf extract after AgNPs synthesis which confirm the role of natural products present in leaves for the conversion of silver ions to AgNPs. The four bright circular rings (111), (200), (220) and (311) observed in the selected area electron diffraction pattern are the characteristic reflections of face centered cubic crystalline silver. LC-MS/MS study revealed the presence of phytochemicals in the leaf extract which is responsible for the reduction of silver ions. MTT assay was performed to investigate the cytotoxicity of AgNPs against two human cell lines, namely HepG2 and WRL-68. The antibacterial study revealed that MIC value of the synthesized AgNPs was 80 µg/ml against Escherichia coli K12 and Staphylococcus aureus (MTCC 96). Finally, the synthesized AgNPs at 10 µg/ml dosages showed beneficial effects on the survivability, body weights of the Bombyx mori L. larvae, pupae, cocoons and shells weights via enhancing the feed efficacy.

Dates et versions

hal-04151000 , version 1 (04-07-2023)

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Sudip Some, Onur Bulut, Kinkar Biswas, Anoop Kumar, Anupam Roy, et al.. Effect of feed supplementation with biosynthesized silver nanoparticles using leaf extract of Morus indica L. V1 on Bombyx mori L. (Lepidoptera: Bombycidae). Scientific Reports, 2019, 9 (1), ⟨10.1038/s41598-019-50906-6⟩. ⟨hal-04151000⟩
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