<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-04139079</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-22T07:34:00+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Microfluidic platform for the reproduction of hypoxic vascular microenvironments</title>
            <author role="aut">
              <persName>
                <forename type="first">Naoyuki</forename>
                <surname>Takahashi</surname>
              </persName>
              <idno type="halauthorid">2843815-0</idno>
              <affiliation ref="#struct-573520"/>
              <affiliation ref="#struct-260631"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Daisuke</forename>
                <surname>Yoshino</surname>
              </persName>
              <idno type="halauthorid">2843816-0</idno>
              <affiliation ref="#struct-411369"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Ryuji</forename>
                <surname>Sugahara</surname>
              </persName>
              <idno type="halauthorid">2843817-0</idno>
              <affiliation ref="#struct-573520"/>
              <affiliation ref="#struct-260631"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Satomi</forename>
                <surname>Hirose</surname>
              </persName>
              <idno type="halauthorid">2274783-0</idno>
              <affiliation ref="#struct-573520"/>
              <affiliation ref="#struct-260631"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Kazuki</forename>
                <surname>Sone</surname>
              </persName>
              <idno type="halauthorid">2843818-0</idno>
              <affiliation ref="#struct-573520"/>
              <affiliation ref="#struct-260631"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">J.-P.</forename>
                <surname>Rieu</surname>
              </persName>
              <email type="md5">d1173a2810f9b501c2dd7a994179257b</email>
              <email type="domain">univ-lyon1.fr</email>
              <idno type="idhal" notation="string">jean-paul-rieu</idno>
              <idno type="idhal" notation="numeric">1133076</idno>
              <idno type="halauthorid" notation="string">428963-1133076</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-0528-8819</idno>
              <idno type="IDREF">https://www.idref.fr/170726363</idno>
              <affiliation ref="#struct-1044299"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Kenichi</forename>
                <surname>Funamoto</surname>
              </persName>
              <idno type="halauthorid">2274785-0</idno>
              <affiliation ref="#struct-573520"/>
              <affiliation ref="#struct-260631"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Armelle</forename>
                <surname>VIDAL</surname>
              </persName>
              <email type="md5">101b9afc274f2ab2d37ca7626243f538</email>
              <email type="domain">univ-lyon1.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2024-03-22 15:48:50</date>
              <date type="whenModified">2026-01-15 16:38:02</date>
              <date type="whenReleased">2024-03-22 15:55:58</date>
              <date type="whenProduced">2023</date>
              <date type="whenEndEmbargoed">2024-03-22</date>
              <ref type="file" target="https://hal.science/hal-04139079v1/document">
                <date notBefore="2024-03-22"/>
              </ref>
              <ref type="file" subtype="greenPublisher" n="1" target="https://hal.science/hal-04139079v1/file/s41598-023-32334-9.pdf" id="file-4517482-3914716">
                <date notBefore="2024-03-22"/>
              </ref>
              <ref type="externalLink" target="https://www.nature.com/articles/s41598-023-32334-9.pdf"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="781428">
                <persName>
                  <forename>Armelle</forename>
                  <surname>VIDAL</surname>
                </persName>
                <email type="md5">101b9afc274f2ab2d37ca7626243f538</email>
                <email type="domain">univ-lyon1.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-04139079</idno>
            <idno type="halUri">https://hal.science/hal-04139079</idno>
            <idno type="halBibtex">takahashi:hal-04139079</idno>
            <idno type="halRefHtml">&lt;i&gt;Scientific Reports&lt;/i&gt;, 2023, 13 (1), pp.5428. &lt;a target="_blank" href="https://dx.doi.org/10.1038/s41598-023-32334-9"&gt;&amp;#x27E8;10.1038/s41598-023-32334-9&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Scientific Reports, 2023, 13 (1), pp.5428. &amp;#x27E8;10.1038/s41598-023-32334-9&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://creativecommons.org/licenses/by/4.0/">CC BY 4.0 - Attribution<ref corresp="#file-4517482-3914716"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-LYON1">Université Claude Bernard - Lyon I</idno>
            <idno type="stamp" n="ILM" corresp="UNIV-LYON1">Institut Lumière Matière</idno>
            <idno type="stamp" n="UDL">UDL</idno>
            <idno type="stamp" n="UNIV-LYON">Université de Lyon</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Microfluidic platform for the reproduction of hypoxic vascular microenvironments</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Naoyuki</forename>
                    <surname>Takahashi</surname>
                  </persName>
                  <idno type="halauthorid">2843815-0</idno>
                  <affiliation ref="#struct-573520"/>
                  <affiliation ref="#struct-260631"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Daisuke</forename>
                    <surname>Yoshino</surname>
                  </persName>
                  <idno type="halauthorid">2843816-0</idno>
                  <affiliation ref="#struct-411369"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Ryuji</forename>
                    <surname>Sugahara</surname>
                  </persName>
                  <idno type="halauthorid">2843817-0</idno>
                  <affiliation ref="#struct-573520"/>
                  <affiliation ref="#struct-260631"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Satomi</forename>
                    <surname>Hirose</surname>
                  </persName>
                  <idno type="halauthorid">2274783-0</idno>
                  <affiliation ref="#struct-573520"/>
                  <affiliation ref="#struct-260631"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Kazuki</forename>
                    <surname>Sone</surname>
                  </persName>
                  <idno type="halauthorid">2843818-0</idno>
                  <affiliation ref="#struct-573520"/>
                  <affiliation ref="#struct-260631"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">J.-P.</forename>
                    <surname>Rieu</surname>
                  </persName>
                  <email type="md5">d1173a2810f9b501c2dd7a994179257b</email>
                  <email type="domain">univ-lyon1.fr</email>
                  <idno type="idhal" notation="string">jean-paul-rieu</idno>
                  <idno type="idhal" notation="numeric">1133076</idno>
                  <idno type="halauthorid" notation="string">428963-1133076</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-0528-8819</idno>
                  <idno type="IDREF">https://www.idref.fr/170726363</idno>
                  <affiliation ref="#struct-1044299"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Kenichi</forename>
                    <surname>Funamoto</surname>
                  </persName>
                  <idno type="halauthorid">2274785-0</idno>
                  <affiliation ref="#struct-573520"/>
                  <affiliation ref="#struct-260631"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">89181</idno>
                <idno type="issn">2045-2322</idno>
                <idno type="eissn">2045-2322</idno>
                <title level="j">Scientific Reports</title>
                <imprint>
                  <publisher>Nature Publishing Group</publisher>
                  <biblScope unit="volume">13</biblScope>
                  <biblScope unit="issue">1</biblScope>
                  <biblScope unit="pp">5428</biblScope>
                  <date type="datePub">2023</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1038/s41598-023-32334-9</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">COLLECTIVE CELL-MIGRATION</term>
                <term xml:lang="en">ENDOTHELIAL-CELLSS</term>
                <term xml:lang="en">HEAR-STRESS</term>
                <term xml:lang="en">TUMOR MICROENVIRONMENT</term>
                <term xml:lang="en">MECHANISMS</term>
                <term xml:lang="en">GUIDANCE</term>
                <term xml:lang="en">CULTURE</term>
                <term xml:lang="en">TIME</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv.bc.bc">Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]</classCode>
              <classCode scheme="halDomain" n="sdv.can">Life Sciences [q-bio]/Cancer</classCode>
              <classCode scheme="halDomain" n="spi.meca.biom">Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]</classCode>
              <classCode scheme="halDomain" n="spi.nano">Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Abstract Vascular endothelial cells (ECs) respond to mechanical stimuli caused by blood flow to maintain vascular homeostasis. Although the oxygen level in vascular microenvironment is lower than the atmospheric one, the cellular dynamics of ECs under hypoxic and flow exposure are not fully understood. Here, we describe a microfluidic platform for the reproduction hypoxic vascular microenvironments. Simultaneous application of hypoxic stress and fluid shear stress to the cultured cells was achieved by integrating a microfluidic device and a flow channel that adjusted the initial oxygen concentration in a cell culture medium. An EC monolayer was then formed on the media channel in the device, and the ECs were observed after exposure to hypoxic and flow conditions. The migration velocity of the ECs immediately increased after flow exposure, especially in the direction opposite to the flow direction, and gradually decreased, resulting in the lowest value under the hypoxic and flow exposure condition. The ECs after 6-h simultaneous exposure to hypoxic stress and fluid shear stress were generally aligned and elongated in the flow direction, with enhanced VE-cadherin expression and actin filament assembly. Thus, the developed microfluidic platform is useful for investigating the dynamics of ECs in vascular microenvironments.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-573520" status="VALID">
          <orgName>Graduate School of Biomedical Engineering [Tohoku University]</orgName>
          <desc>
            <address>
              <addrLine>6-6-12, Aramaki Aza Aoba Aoba-ku, Sendai, Miyagi 980-8579, Japan</addrLine>
              <country key="JP"/>
            </address>
            <ref type="url">https://www.bme.tohoku.ac.jp/english/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-311564" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-260631" status="VALID">
          <orgName>Institute of Fluid Science [Tohoku University]</orgName>
          <desc>
            <address>
              <addrLine>2 Chome-1-1 Katahira, Aoba Ward, Sendai, Miyagi 980-0812</addrLine>
              <country key="JP"/>
            </address>
            <ref type="url">https://www.ifs.tohoku.ac.jp/eng/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-311564" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-411369" status="VALID">
          <orgName>Tokyo University of Agriculture and Technology</orgName>
          <orgName type="acronym">TUAT</orgName>
          <desc>
            <address>
              <country key="JP"/>
            </address>
            <ref type="url">http://www.tuat.ac.jp/en/</ref>
          </desc>
        </org>
        <org type="researchteam" xml:id="struct-1044299" status="VALID">
          <orgName>iLM - Biophysique</orgName>
          <orgName type="acronym">iLM - BIOPHYSIQUE</orgName>
          <date type="start">2013-01-01</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://ilm.univ-lyon1.fr/index.php?option=com_content&amp;view=article&amp;id=770&amp;catid=41</ref>
          </desc>
          <listRelation>
            <relation active="#struct-224716" type="direct"/>
            <relation active="#struct-194495" type="indirect"/>
            <relation active="#struct-301088" type="indirect"/>
            <relation name="UMR5306" active="#struct-441569" type="indirect"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-311564" status="VALID">
          <idno type="ROR">https://ror.org/01dq60k83</idno>
          <orgName>Tohoku University [Sendai]</orgName>
          <desc>
            <address>
              <addrLine>2-1-1 Katahira, Aoba-ku, Sendai, 980-8577 Japan</addrLine>
              <country key="JP"/>
            </address>
            <ref type="url">https://www.tohoku.ac.jp/en/</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-224716" status="VALID">
          <idno type="IdRef">17806890X</idno>
          <idno type="ISNI">0000000403844911</idno>
          <idno type="RNSR">201320570G</idno>
          <idno type="ROR">0323bey33</idno>
          <orgName>Institut Lumière Matière [Villeurbanne]</orgName>
          <orgName type="acronym">ILM</orgName>
          <date type="start">2013-01-01</date>
          <desc>
            <address>
              <addrLine>UMR5306 CNRS Université Claude Bernard Lyon 1 Domaine Scientifique de La Doua Bâtiment Kastler, 10 rue Ada Byron 69622 Villeurbanne CEDEX, Franc</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://ilm.univ-lyon1.fr/index.php</ref>
          </desc>
          <listRelation>
            <relation active="#struct-194495" type="direct"/>
            <relation active="#struct-301088" type="indirect"/>
            <relation name="UMR5306" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-194495" status="VALID">
          <idno type="IdRef">026402823</idno>
          <idno type="ISNI">0000000121686185</idno>
          <idno type="ROR">https://ror.org/029brtt94</idno>
          <orgName>Université Claude Bernard Lyon 1</orgName>
          <orgName type="acronym">UCBL</orgName>
          <desc>
            <address>
              <addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-lyon1.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-301088" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-301088" status="VALID">
          <idno type="ROR">https://ror.org/01rk35k63</idno>
          <orgName>Université de Lyon</orgName>
          <desc>
            <address>
              <addrLine>92 rue Pasteur - CS 30122, 69361 Lyon Cedex 07</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.universite-lyon.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>