Water covers about 71 percent of the Earth’s surface (i.e., oceans, glaciers) and it is crucial for all the biological systems. Although bulk water is inert, water microdroplets provide a favorablefavourable environment for chemical processes. The investigation and the understanding of the physico-chemical processes that occur in the atmospheric aerosols is of great importance, while aerosols is well-known that have an adverse effect in air-quality, climate and public health. In the air-water interface the presence of a strong electric field can lead to the acceleration of chemical reactions and initiate spontaneous reduction of organic compounds. Our study is focusing on the spontaneous H2O2 production in at the interface of water droplets, which occurs via the recombination of the hydroxyl radicals that are formed via the dissociation of hydroxyl ions, while other pathways cannot be excluded. H2O2 may play a key role in the oxidation of atmospheric aerosols and therefore, it may alter the oxidation capacity by increasing the production of radicals. Within this framework, a thorough laboratory study, using state-of-the-art instrumentation has been carried out. Two different types of experiments were performed, where the H2O2 and thus the OH production was measured either directly or indirectly by using sensitive water-soluble fluorescent probes. Aqueous microdroplets, in a range of diameter 0.1 to 10 µm were generated by nebulizing salted solutions inside a glass reactor. These particles were then collected after 4 hours reaction time and the liquid phase H2O2 was measured by using an H2O2 analyser. During our experiments, an Optical Particle Counter was connected in order to monitor the size distribution and the number of the particles. To extend our understanding in the processes that occur atin the interface, different types of salts were selected (NH4Cl, Na2SO4 and CaSO4) in order to investigate the way that different ions of different valence affect the H2O2 production. A correlation between the size distribution and the hydrogen peroxide concentration was also performed. In order to verify the OH production, salted solutions containing terephalic acid (TA) were also nebulised inside the reactor. The collected droplets were analysed via fluorescent spectroscopy where the 2-hydroxyterephthalic acid (TAOH), product of the reaction of TA with OH radicals, was observed. TAOH was also observed in the particle phase in a size range of 1–5 µm. All the experiments provide evidence that H2O2 is produced in the air-water interface of microdroplets at a range of (1–7)×10-2 µM, which depends on the size distribution, the concentration of the solution and the type of salt. Results from this study are expected to significantly improve our insight on the processes that occur in atmospheric droplets and to assess the contribution of this OH radical source in total atmospheric budget.