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Poster De Conférence Année : 2023

Untargeted metabolomics on dried blood spots

Métabolomique non ciblée sur gouttes de sang séché

Résumé

Blood micro-sampling was initially used in hospitals to screen newborns for congenital diseases; today, this type of sampling is increasingly used in clinical studies. Analysed mainly using targeted methods, few studies report results using an open metabolomics approach. The aim of the work presented here is to evaluate sampling devices, extraction methods for these samples and the analytical coverage obtained by LCHRMS. Whatman 903® collection papers, mainly used for these samples, and Mitra® Neoteryx supports with a fixed sample volume of 20µL, were tested under different storage and extraction conditions. Analyses were carried out using heparinised plasma and whole blood on LC-QToF, then reprocessed on W4M. Initial results enabled a qualitative comparison to be made between heparinised plasma samples and the same samples deposited on the media studied under the different conditions. After optimising the preparation of this type of sample, the whole blood samples could be characterised. In conclusion, this preliminary work in open metabolomics will make it possible to investigate the analysis of micro-samples, a minimally invasive collection method that is easy to use at home, to answer questions relating to clinical studies and the development of participatory science.
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Dates et versions

hal-04128127 , version 1 (14-06-2023)

Identifiants

  • HAL Id : hal-04128127 , version 1

Citer

Marie Lagrée, Antoine Mercier, Estelle Pujos-Guillot, Stéphanie Durand. Untargeted metabolomics on dried blood spots. 15èmes journées scientifiques du RFMF, May 2023, Perpignan, France. ⟨hal-04128127⟩
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