In this contribution we focus on a portion of the Mugesse mega shear zone (MGSZ) in the Ubendian Belt, for which details on kinematics and strain partitioning remains poorly documented. We present a field-based structural work, a petrological and thermobarometrical study of mylonites and LA-ICP-MS in-situ U-Pb dating on monazite and zircon. The detailed field mapping highlights that the whole study area shows the same sinistral strain pattern with deformation being partitioned within S-C and C-C’ structures. Syn-kinematic metamorphic mineral assemblages indicate that the MGSZ mylonites recorded a clockwise P-T evolution with peak temperature conditions at upper amphibolite metamorphic facies of 11–15 kbar and 760–790 °C. Retrogression and cooling reached P-T conditions of 1–7 kbar and 330–480 °C. LA-ICP-MS in-situ U-Pb monazite dating suggests that the main activity of the MGSZ was Pan-African, at ca. 547 ± 5 and 545 ± 4 Ma. Zircon dates at 1985 ± 44 Ma and 1127 ± 29–988 ± 10 Ma are interpreted as inherited ages of potentially detrital origin related to the Ubendian and Irumide orogeny, respectively. A 1146 ± 14 Ma date obtained on zircons from the Lwakwa mylonitic orthogneiss is interpreted as the crystallisation age of the granitic protolith that intruded the Paleoproterozoic basement. Strain partitioning and strain softening during the growth of the MGSZ was strongly controlled by feedback relationships between deformation and retrograde metamorphism enhanced by fluid ingress and recrystallisation.