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Pré-Publication, Document De Travail Année : 2023

A novel injectable radiopaque hydrogel with potent properties for multicolor CT imaging in the context of brain and cartilage regenerative therapy

Karine Toupet
Yuxi Clara Dong
Nora Collomb
  • Fonction : Auteur
Céline Auxenfans
Anaïck Moisan
  • Fonction : Auteur
Bertrand Favier
Benoit Chovelon
Emmanuel Luc Barbier
Christian Jorgensen
David Peter Cormode
Danièle Noël
Emmanuel Brun
Hélène Elleaume
Olivier Detante
Claire Rome
Rachel Auzély-Velty

Résumé

Cell therapy is promising to treat many conditions, including neurological and osteoarticular diseases. Encapsulation of cells within hydrogels facilitates cell delivery and can improve therapeutic effects. However, much work remains to be done to align treatment strategies with specific diseases. The development of imaging tools that enable monitoring cells and hydrogel independently is key to achieving this goal. Our objective herein is to longitudinally study an iodine-labeled hydrogel, incorporating gold-labeled stem cells, by bicolor CT imaging after in vivo injection in rodent brains or knees. To this aim, an injectable self-healing hyaluronic acid (HA) hydrogel with long-persistent radiopacity was formed by the covalent grafting of a clinical contrast agent on HA. The labeling conditions were tuned to achieve sufficient X-ray signal and to maintain the mechanical and self-healing properties as well as injectability of the original HA scaffold. The efficient delivery of both cells and hydrogel at the targeted sites was demonstrated by synchrotron K-edge subtraction-CT. The iodine labeling enabled to monitor the hydrogel biodistribution in vivo up to 3 days post-administration, which represents a technological first in the field of molecular CT imaging agents. This tool may foster the translation of combined cell-hydrogel therapies into the clinics.
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Dates et versions

hal-04126192 , version 1 (13-06-2023)

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Moustoifa Said, Clément Tavakoli, Chloé Dumot, Karine Toupet, Yuxi Clara Dong, et al.. A novel injectable radiopaque hydrogel with potent properties for multicolor CT imaging in the context of brain and cartilage regenerative therapy. 2023. ⟨hal-04126192⟩
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