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Article Dans Une Revue Cell Reports Année : 2023

Ovulation is triggered by a cyclical modulation of gonadotropes into a hyperexcitable state

Résumé

Gonadotropes in the anterior pituitary gland are essential for fertility and provide a functional link between the brain and the gonads. To trigger ovulation, gonadotrope cells release massive amounts of luteinizing hormone (LH). The mechanism underlying this remains unclear. Here, we utilize a mouse model expressing a genetically encoded Ca2+ indicator exclusively in gonadotropes to dissect this mechanism in intact pituitaries. We demonstrate that female gonadotropes exclusively exhibit a state of hyperexcitability during the LH surge, resulting in spontaneous [Ca2+]i transients in these cells, which persist in the absence of any in vivo hormonal signals. L-type Ca2+ channels and transient receptor potential channel A1 (TRPA1) together with intracellular reactive oxygen species (ROS) levels ensure this state of hyperexcitability. Consistent with this, virus-assisted triple knockout of Trpa1 and L-type Ca2+ subunits in gonadotropes leads to vaginal closure in cycling females. Our data provide insight into molecular mechanisms required for ovulation and reproductive success in mammals.
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hal-04121438 , version 1 (07-11-2023)

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Viktoria Götz, Sen Qiao, Debajyoti Das, Philipp Wartenberg, Amanda Wyatt, et al.. Ovulation is triggered by a cyclical modulation of gonadotropes into a hyperexcitable state. Cell Reports, 2023, 42 (6), pp.112543. ⟨10.1016/j.celrep.2023.112543⟩. ⟨hal-04121438⟩
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