Anti-Herpes simplex virus (HSV-1) activity and antioxidant capacity of carrageenan-rich enzymatic extracts from Solieria filiformis (Gigartinales, Rhodophyta)
Résumé
Solieria filiformis has been reported to havemolecules with various biological activities. In this studywe used environmentally
friendly extractionmethods, such as enzyme-assisted extraction (EAE), as a first step to obtain bioactive
compounds fromthis species. Five combinations of protease (PRO) and carbohydrase (AMG)were utilized
(1:0, 0:1, 2:1, 1:1, 1:2 PRO:AMG) to obtain Water Soluble Enzymatic Hydrolysates (WSEHs). Extraction yields,
biochemical and structural characterization, as well as in vitro activity against Herpes simplex virus type 1
(HSV-1) and antioxidant capacities were determined. All PRO:AMG combinations significantly improved yields.
EAE yielded heterogeneous extracts rich in iota-carrageenan and phenols, as confirmed by FTIR spectra. The
highest antiherpetic activity (EC50 4.5 ± 0.4 μg mL−1) was found in the WSEHs obtained under 2:1 PRO:AMG.
At this combination high antioxidant capacity was also obtained for ABTS (2,2′-Azino-Bis-3-ethylbenzoThiazoline-
6-Sulfonic acid) radical scavenging activity and Ferric Reducing Antioxidant Power (FRAP). These could
probably play a synergistic role associated to the strong antiviral activity obtained. These results suggest that
2:1 PRO:AMGcould be effective in promoting the hydrolytic breakdown of highMWpolysaccharides, contributing
to the improvement ofWSEHs bioactivity. Although Solieria filiformis WSEHs showed promising results, further
research, including separation and purification techniques are needed.