Anti-Herpes simplex virus (HSV-1) activity and antioxidant capacity of carrageenan-rich enzymatic extracts from Solieria filiformis (Gigartinales, Rhodophyta) - Archive ouverte HAL
Article Dans Une Revue International Journal of Biological Macromolecules Année : 2021

Anti-Herpes simplex virus (HSV-1) activity and antioxidant capacity of carrageenan-rich enzymatic extracts from Solieria filiformis (Gigartinales, Rhodophyta)

Résumé

Solieria filiformis has been reported to havemolecules with various biological activities. In this studywe used environmentally friendly extractionmethods, such as enzyme-assisted extraction (EAE), as a first step to obtain bioactive compounds fromthis species. Five combinations of protease (PRO) and carbohydrase (AMG)were utilized (1:0, 0:1, 2:1, 1:1, 1:2 PRO:AMG) to obtain Water Soluble Enzymatic Hydrolysates (WSEHs). Extraction yields, biochemical and structural characterization, as well as in vitro activity against Herpes simplex virus type 1 (HSV-1) and antioxidant capacities were determined. All PRO:AMG combinations significantly improved yields. EAE yielded heterogeneous extracts rich in iota-carrageenan and phenols, as confirmed by FTIR spectra. The highest antiherpetic activity (EC50 4.5 ± 0.4 μg mL−1) was found in the WSEHs obtained under 2:1 PRO:AMG. At this combination high antioxidant capacity was also obtained for ABTS (2,2′-Azino-Bis-3-ethylbenzoThiazoline- 6-Sulfonic acid) radical scavenging activity and Ferric Reducing Antioxidant Power (FRAP). These could probably play a synergistic role associated to the strong antiviral activity obtained. These results suggest that 2:1 PRO:AMGcould be effective in promoting the hydrolytic breakdown of highMWpolysaccharides, contributing to the improvement ofWSEHs bioactivity. Although Solieria filiformis WSEHs showed promising results, further research, including separation and purification techniques are needed.
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Dates et versions

hal-04104378 , version 1 (24-05-2023)

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Peñuela Ana, Bourgougnon Nathalie, Bedoux Gilles, Robledo Daniel, Madera-Santana Tomás, et al.. Anti-Herpes simplex virus (HSV-1) activity and antioxidant capacity of carrageenan-rich enzymatic extracts from Solieria filiformis (Gigartinales, Rhodophyta). International Journal of Biological Macromolecules, 2021, 168, pp.322-330. ⟨10.1016/j.ijbiomac.2020.12.064⟩. ⟨hal-04104378⟩
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