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Article Dans Une Revue STAR Protocols Année : 2023

Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy

Résumé

Stiffness plays a central action in plant cell extension. Here, we present a protocol to detect changes in stiffness on the external epidermal cell wall of living plant roots using atomic force microscopy (AFM). We provide generalized instructions for collecting force-distance curves and analysis of stiffness using contact-based mechanical model. With this protocol, and some initial training in AFM, a user is able to perform indentation experiments on 4-and 5-day-old Arabidopsis thaliana and determine stiffness properties.
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Origine : Publication financée par une institution
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-04102900 , version 1 (22-05-2023)

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Harinderbir Kaur, Jean-Marie Teulon, Anne-Emmanuelle Foucher, Daphna Fenel, Shu-Wen W Chen, et al.. Measuring external primary cell wall elasticity of seedling roots using atomic force microscopy. STAR Protocols, 2023, 4 (2), pp.102265. ⟨10.1016/j.xpro.2023.102265⟩. ⟨hal-04102900⟩
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