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Article Dans Une Revue eLife Année : 2022

Viscoelastic properties of suspended cells measured with shear flow deformation cytometry

Mar Eroles
Jennifer Elsterer
  • Fonction : Auteur
Astrid Mainka
  • Fonction : Auteur
Selina Sonntag
  • Fonction : Auteur
Johannes Bartl
  • Fonction : Auteur
Lena Fischer
  • Fonction : Auteur
Shada Abuhattum
  • Fonction : Auteur
Ruchi Goswami
  • Fonction : Auteur
Salvatore Girardo
  • Fonction : Auteur
Stefan Schrüfer
  • Fonction : Auteur
Nadine Ströhlein
  • Fonction : Auteur
Mojtaba Nosratlo
  • Fonction : Auteur
Felix Rico
Sebastian Johannes Müller
Stephan Gekle
  • Fonction : Auteur
Ben Fabry

Résumé

Numerous cell functions are accompanied by phenotypic changes in viscoelastic properties, and measuring them can help elucidate higher level cellular functions in health and disease. We present a high-throughput, simple and low-cost microfluidic method for quantitatively measuring the elastic (storage) and viscous (loss) modulus of individual cells. Cells are suspended in a high-viscosity fluid and are pumped with high pressure through a 5.8 cm long and 200 µm wide microfluidic channel. The fluid shear stress induces large, ear ellipsoidal cell deformations. In addition, the flow profile in the channel causes the cells to rotate in a tank-treading manner. From the cell deformation and tank treading frequency, we extract the frequency-dependent viscoelastic cell properties based on a theoretical framework developed by R. Roscoe [1] that describes the deformation of a viscoelastic sphere in a viscous fluid under steady laminar flow. We confirm the accuracy of the method using atomic force microscopy-calibrated polyacrylamide beads and cells. Our measurements demonstrate that suspended cells exhibit power-law, soft glassy rheological behavior that is cell-cycle-dependent and mediated by the physical interplay between the actin filament and intermediate filament networks.
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Dates et versions

hal-04100953 , version 1 (20-02-2024)

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Paternité

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Richard Gerum, Elham Mirzahossein, Mar Eroles, Jennifer Elsterer, Astrid Mainka, et al.. Viscoelastic properties of suspended cells measured with shear flow deformation cytometry. eLife, 2022, 11, ⟨10.7554/eLife.78823⟩. ⟨hal-04100953⟩
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