Characterization of the proneural gene regulatory network during mouse telencephalon development - Archive ouverte HAL Access content directly
Journal Articles BMC Biology Year : 2008

Characterization of the proneural gene regulatory network during mouse telencephalon development

Julia M. Gohlke
  • Function : Author
Olivier Armant
  • Function : Author
Frederick M. Parham
  • Function : Author
Marjolein V. Smith
  • Function : Author
Celine Zimmer
  • Function : Author
Diogo S. Castro
  • Function : Author
Laurent Nguyen
  • Function : Author
Joel S. Parker
  • Function : Author
Christopher J. Portier
  • Function : Author
François Guillemot
  • Function : Author

Abstract

BACKGROUND: The proneural proteins Mash1 and Ngn2 are key cell autonomous regulators of neurogenesis in the mammalian central nervous system, yet little is known about the molecular pathways regulated by these transcription factors. RESULTS: Here we identify the downstream effectors of proneural genes in the telencephalon using a genomic approach to analyze the transcriptome of mice that are either lacking or overexpressing proneural genes. Novel targets of Ngn2 and/or Mash1 were identified, such as members of the Notch and Wnt pathways, and proteins involved in adhesion and signal transduction. Next, we searched the non-coding sequence surrounding the predicted proneural downstream effector genes for evolutionarily conserved transcription factor binding sites associated with newly defined consensus binding sites for Ngn2 and Mash1. This allowed us to identify potential novel co-factors and co-regulators for proneural proteins, including Creb, Tcf/Lef, Pou-domain containing transcription factors, Sox9, and Mef2a. Finally, a gene regulatory network was delineated using a novel Bayesian-based algorithm that can incorporate information from diverse datasets. CONCLUSION: Together, these data shed light on the molecular pathways regulated by proneural genes and demonstrate that the integration of experimentation with bioinformatics can guide both hypothesis testing and hypothesis generation.
Fichier principal
Vignette du fichier
islandora_162899.pdf (3.19 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive

Dates and versions

hal-04098272 , version 1 (15-05-2023)

Identifiers

Cite

Julia M. Gohlke, Olivier Armant, Frederick M. Parham, Marjolein V. Smith, Celine Zimmer, et al.. Characterization of the proneural gene regulatory network during mouse telencephalon development. BMC Biology, 2008, 6, ⟨10.1186/1741-7007-6-15⟩. ⟨hal-04098272⟩
13 View
9 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More