Article Dans Une Revue Development (Cambridge, England) Année : 2000

Basic helix-loop-helix transcription factors regulate the neuroendocrine differentiation of fetal mouse pulmonary epithelium

Takaaki Ito
  • Fonction : Auteur
Naoko Udaka
  • Fonction : Auteur
Takuya Yazawa
  • Fonction : Auteur
Koji Okudela
  • Fonction : Auteur
Hiroshi Hayashi
  • Fonction : Auteur
Tetsuo Sudo
  • Fonction : Auteur
Ryoichiro Kageyama
  • Fonction : Auteur
Hitoshi Kitamura
  • Fonction : Auteur

Résumé

ABSTRACT To clarify the mechanisms that regulate neuroendocrine differentiation of fetal lung epithelia, we have studied the expression of the mammalian homologs of achaete-scute complex (Mash1) (Ascl1 – Mouse Genome Informatics); hairy and enhancer of split1 (Hes1); and the expression of Notch/Notch-ligand system in the fetal and adult mouse lungs, and in the lungs of Mash1- or Hes1-deficient mice. Immunohistochemical studies revealed that Mash1-positive cells seemed to belong to pulmonary neuroendocrine cells (PNEC) and their precursors. In mice deficient for Mash1, no PNEC were detected. Hes1-positive cells belong to non-neuroendocrine cells. In the mice deficient in Hes1, in which Mash1 mRNA was upregulated, PNEC appeared precociously, and the number of PNEC was markedly increased. NeuroD (Neurod1 – Mouse Genome Informatics) expression in the lung was detected in the adult, and was enhanced in the fetal lungs of Hes1-null mice. Expression of Notch1, Notch2, Notch3 and Notch4 mRNAs in the mouse lung increased with age, and Notch1 mRNA was expressed in a Hes1-dependent manner. Notch1, Notch2 and Notch3 were immunohistochemically detected in non-neuroendocrine cells. Moreover, analyses of the lungs from the gene-targeted mice suggested that expression of Delta-like 1 (Dll1 – Mouse Genome Informatics) mRNA depends on Mash1. Thus, the neuroendocrine differentiation depends on basic helix-loop-helix factors, and Notch/Notch-ligand pathways may be involved in determining the cell differentiation fate in fetal airway epithelium.

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Dates et versions

hal-04093722 , version 1 (10-05-2023)

Identifiants

Citer

Takaaki Ito, Naoko Udaka, Takuya Yazawa, Koji Okudela, Hiroshi Hayashi, et al.. Basic helix-loop-helix transcription factors regulate the neuroendocrine differentiation of fetal mouse pulmonary epithelium. Development (Cambridge, England), 2000, 127 (18), pp.3913-3921. ⟨10.1242/dev.127.18.3913⟩. ⟨hal-04093722⟩
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