Redox-Active Bis-Cyclometalated Iridium(III) Complex as a DNA Photo-Cleaving Agent
Résumé
The development of new photoactive metal
complexes that can trigger oxidative damages to the genetic
material is of great interest. In the present paper, we describe the
detailed study of a highly photo-oxidant iridium(III) complex that
triggers photoinduced electron transfer (PET) with purine DNA
bases. The PET has been studied by luminescence and laser flash
photolysis experiments. From plasmid DNA agarose gel electrophoresis
experiments, we demonstrated the high ability of the
iridium complex to induce strand breaks upon light irradiation.
Reactive oxygen species (ROS)-specific scavengers and stabilizers
were employed to identify that the photocleavage process, the
results of which infer singlet oxygen and hydrogen peroxide as the
predominant species. To the best of our knowledge, the present
work represents one of the few study for highly photo-oxidant bis-cyclometalated iridium(III) complex toward DNA.